CRISPR-Cas9: A method for establishing rat models of drug metabolism and pharmacokinetics
| العنوان: | CRISPR-Cas9: A method for establishing rat models of drug metabolism and pharmacokinetics |
|---|---|
| المؤلفون: | Yuanjin Zhang, Yuanqing Guo, Yeye Xu, Jie Liu, Jian Lu, Xin Wang |
| المصدر: | Acta Pharmaceutica Sinica B, Vol 11, Iss 10, Pp 2973-2982 (2021) Acta Pharmaceutica Sinica. B |
| بيانات النشر: | Elsevier, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | NCBI, National Center for Biotechnology Information, TALEN, transcriptional activators like effector nucleases, RNP, ribonucleoprotein, Review, Gene editing, SREBP-2, sterol regulatory element-binding protein 2, 0302 clinical medicine, AAV, adeno-associated virus, Genome editing, DDI, drug–drug interaction, CRISPR, General Pharmacology, Toxicology and Pharmaceutics, 0303 health sciences, HDR, homology directed repair, tracRNA, trans-activating crRNA, ADMET, absorption, distribution, metabolism, excretion and toxicity, HIV, human immunodeficiency virus, 030220 oncology & carcinogenesis, CRISPR-Cas9, OATP1B, organic anion transporting polypeptides 1B, Rat model, CRISPR-Cas, clustered regularly interspaced short palindromic repeats-CRISPR-associated, pre-crRNA, pre-CRISPR RNA, Computational biology, RM1-950, 03 medical and health sciences, Animal model, Pharmacokinetics, TALE, transcriptional activator-like effector, ZFN, zinc finger nucleases, DSB, double-strand break, PAM, protospacer-associated motif, Gene knockout, BSEP, bile salt export pump, 030304 developmental biology, Drug metabolism, KO, knockout, Mechanism (biology), sgRNA, single guide RNA, crRNAs, CRISPR RNAs, WT, wild-type, HBV, hepatitis B virus, T7E I, T7 endonuclease I, NHEJ, non-homologous end joining, SD, Sprague–Dawley, OTS, off-target site, DMPK, drug metabolism and pharmacokinetics, Therapeutics. Pharmacology, HPV, human papillomaviruses |
| الوصف: | The 2020 Nobel Prize in Chemistry recognized CRISPR-Cas9, a super-selective and precise gene editing tool. CRISPR-Cas9 has an obvious advantage in editing multiple genes in the same cell, and presents great potential in disease treatment and animal model construction. In recent years, CRISPR-Cas9 has been used to establish a series of rat models of drug metabolism and pharmacokinetics (DMPK), such as Cyp, Abcb1, Oatp1b2 gene knockout rats. These new rat models are not only widely used in the study of drug metabolism, chemical toxicity, and carcinogenicity, but also promote the study of DMPK related mechanism, and further strengthen the relationship between drug metabolism and pharmacology/toxicology. This review systematically introduces the advantages and disadvantages of CRISPR-Cas9, summarizes the methods of establishing DMPK rat models, discusses the main challenges in this field, and proposes strategies to overcome these problems. Graphical abstract CRISPR-Cas9 has been applied to construct rat models of drug metabolism and pharmacokinetics (DMPK). These new models are used not only to study drug metabolism and transport, but also to explore the mechanism of DMPK.Image 1 |
| اللغة: | English |
| تدمد: | 2211-3835 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::953da916a138cd7d6b71c2e8c8f9668aTest http://www.sciencedirect.com/science/article/pii/S2211383521000113Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....953da916a138cd7d6b71c2e8c8f9668a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22113835 |
|---|