Konjac ceramide (kCer) regulates keratinocyte migration by Sema3A-like repulsion mechanism
| العنوان: | Konjac ceramide (kCer) regulates keratinocyte migration by Sema3A-like repulsion mechanism |
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| المؤلفون: | Susumu Mitsutake, Seigo Usuki, Yasuyuki Igarashi, Junken Aoki, Asuka Inoue, Kunikazu Tanji, Katsuyuki Mukai, Shigeki Higashiyama, Noriko Tamura, Tomohiro Tamura |
| المصدر: | Biochemistry and Biophysics Reports Biochemistry and Biophysics Reports, Vol 17, Iss, Pp 132-138 (2019) |
| بيانات النشر: | Elsevier, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Ceramide, Neurite, GlcCer, glucosylceramide, NGF, nerve growth factor, C18Cer, N-octadecanoyl-D-erythro-sphingosine, Biophysics, EGCase I, endoglycoceramidase I, PBS, phosphate-buffered saline, Cer, ceramide, kCer, konjac ceramide, Biochemistry, lcsh:Biochemistry, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, AP-Sema3A, alkaline phosphatase-fused Sema3A, CRMP2, collapsin response mediator protein 2, lcsh:QD415-436, H4R, histamine G-coupled receptor 4, HRs, histamine G-coupled receptors, Keratinocyte migration, lcsh:QH301-705.5, H1R, histamine G-coupled receptor 1, C24Cer, N-tetracosanoyl-D-erythro-sphingosine, p-CRMP2, phospho-collapsin response mediator protein 2, GAPDH, Glyceraldehyde 3-phosphate dehydrogenase, DMEM, Dulbecco's modified Eagle's medium, C16Cer, N-hexadecanoyl-D-erythro-sphingosine, Cell migration, hGPCR, histamine G-coupled receptor, Cofilin, Cell biology, HaCaT, 030104 developmental biology, lcsh:Biology (General), chemistry, 030220 oncology & carcinogenesis, Sema3A, semaphorin 3A, Phosphorylation, BSA, bovine serum albumin, Signal transduction, Nrp1, neuropilin1, p-Cofilin, phospho-Cofilin, His, histamine, Research Article |
| الوصف: | Previously, we proposed the following mechanism for konjac ceramide (kCer)-mediated neurite outgrowth inhibition: kCer binds to Nrp as a Sema3A agonist, resulting in Nrp1/PlexA complex formation and activation of the Sema3A signaling pathway to induce phosphorylation of CRMP2 and microtubule depolymerization. The Sema3A/Nrp1 signaling pathway is known to be also expressed in normal human keratinocytes. To determine whether kCer can function in human keratinocytes as it does in neurites, that is, if it can bind to Nrp1 in place of Sema3A, we studied the effect of kCer on HaCaT cell migration activity. Using a trans-well chamber assay, we compared the effects of Sema3A and kCer on serum-derived cell migration activity. kCer showed Sema3A-like suppression of cell migration activity and induction of cellular Cofilin phosphorylation. In addition, kCer and Sema3A inhibited histamine (His)-enhanced migration of immature HaCaT cells. We have demonstrated that kCer does not interact with histaime receptors H1R or H4R directly, but we speculate that kCer may transduce a signal downstream of the His signaling pathway. Highlights • Konjac ceramide (kCer) inhibits HaCaT cell migration by Sema3A-like action. • kCer displaces the binding of Sema3A to cell surface receptor. • kCer inhibits histamine-induced stimulation of HaCaT cell migration. • kCer does not interact with histamine receptors, H1R and H4R. |
| اللغة: | English |
| تدمد: | 2405-5808 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::848a41fe1fa7b6f0e58885cec5e2bf17Test http://europepmc.org/articles/PMC6319021Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....848a41fe1fa7b6f0e58885cec5e2bf17 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 24055808 |
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