التفاصيل البيبلوغرافية
| العنوان: |
In search of an evidence-based strategy for quality assessment of human tissue samples: report of the tissue biospecimen research working group of the spanish biobank network |
| المؤلفون: |
Esteva‑Socias, M. (Margalida), Artiga, M.J. (María Jesús), Bahamonde, O. (Olga), Belar, O. (Oihana), Raquel, Castro, E. (Erika), Escámez, T. (Teresa), Fraga, M. (Máximo), Jauregui‑Mosquera, L. (Laura), Novoa, I. (Isabel), Peiró‑Chova, L. (Lorena), Rejón, J.D. (Juan David), Ruiz‑Miró, M. (María), Vieiro-Balo, P. (Paula), Villar-Campo, V. (Virginia), Zazo, S. (Sandra), Rábano, A. (Alberto), Villena, C. (Cristina) |
| بيانات النشر: |
Springer Science and Business Media LLC |
| سنة النشر: |
2019 |
| المجموعة: |
dadun - Depósito Académico Digital Universidad de Navarra |
| مصطلحات موضوعية: |
Quality, Pre-analytical variables, Biobank, Tissue, Biospecimen science |
| الوصف: |
The purpose of the present work is to underline the importance of obtaining a standardized procedure to ensure and evaluate both clinical and research usability of human tissue samples. The study, which was carried out by the Biospecimen Science Working Group of the Spanish Biobank Network, is based on a general overview of the cur‑ rent situation about quality assurance in human tissue biospecimens. It was conducted an exhaustive review of the analytical techniques used to evaluate the quality of human tissue samples over the past 30 years, as well as their reference values if they were published, and classifed them according to the biomolecules evaluated: (i) DNA, (ii) RNA, and (iii) soluble or/and fxed proteins for immunochemistry. More than 130 publications released between 1989 and 2019 were analysed, most of them reporting results focused on the analysis of tumour and biopsy samples. A qual‑ ity assessment proposal with an algorithm has been developed for both frozen tissue samples and formalin-fxed parafn-embedded (FFPE) samples, according to the expected quality of sample based on the available pre-analytical information and the experience of the participants in the Working Group. The high heterogeneity of human tissue samples and the wide number of pre-analytic factors associated to quality of samples makes it very difcult to harmo‑ nize the quality criteria. However, the proposed method to assess human tissue sample integrity and antigenicity will not only help to evaluate whether stored human tissue samples ft for the purpose of biomarker development, but will also allow to perform further studies, such as assessing the impact of diferent pre-analytical factors on very well characterized samples or evaluating the readjustment of tissue sample collection, processing and storing procedure |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
https://hdl.handle.net/10171/62538Test |
| الإتاحة: |
https://hdl.handle.net/10171/62538Test |
| حقوق: |
info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edsbas.DC76F97E |
| قاعدة البيانات: |
BASE |
