دورية أكاديمية
Electrical Stimulation of Mesenchymal Stem Cells as a Tool for Proliferation and Differentiation in Cartilage Tissue Engineering: A Scaffold-Based Approach
| العنوان: | Electrical Stimulation of Mesenchymal Stem Cells as a Tool for Proliferation and Differentiation in Cartilage Tissue Engineering: A Scaffold-Based Approach |
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| المؤلفون: | Nicolas Lehmenkötter, Johannes Greven, Frank Hildebrand, Philipp Kobbe, Jörg Eschweiler |
| المصدر: | Bioengineering, Vol 11, Iss 6, p 527 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Technology LCC:Biology (General) |
| مصطلحات موضوعية: | electrical stimulation, mesenchymal stem cells, osteoarthritis, scaffold, cartilage, chondrogenesis, Technology, Biology (General), QH301-705.5 |
| الوصف: | Electrical stimulation (ES) is a widely discussed topic in the field of cartilage tissue engineering due to its ability to induce chondrogenic differentiation (CD) and proliferation. It shows promise as a potential therapy for osteoarthritis (OA). In this study, we stimulated mesenchymal stem cells (MSCs) incorporated into collagen hydrogel (CH) scaffolds, consisting of approximately 500,000 cells each, for 1 h per day using a 2.5 Vpp (119 mV/mm) 8 Hz sinusoidal signal. We compared the cell count, morphology, and CD on days 4, 7, and 10. The results indicate proliferation, with an increase ranging from 1.86 to 9.5-fold, particularly on day 7. Additionally, signs of CD were observed. The stimulated cells had a higher volume, while the stimulated scaffolds showed shrinkage. In the ES groups, up-regulation of collagen type 2 and aggrecan was found. In contrast, SOX9 was up-regulated in the control group, and MMP13 showed a strong up-regulation, indicating cell stress. In addition to lower stress levels, the control groups also showed a more spheroidic shape. Overall, scaffold-based ES has the potential to achieve multiple outcomes. However, finding the appropriate stimulation pattern is crucial for achieving successful chondrogenesis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2306-5354 |
| العلاقة: | https://www.mdpi.com/2306-5354/11/6/527Test; https://doaj.org/toc/2306-5354Test |
| DOI: | 10.3390/bioengineering11060527 |
| الوصول الحر: | https://doaj.org/article/2f6e533d148f4636b29677e77cb0a70cTest |
| رقم الانضمام: | edsdoj.2f6e533d148f4636b29677e77cb0a70c |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23065354 |
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| DOI: | 10.3390/bioengineering11060527 |