دورية أكاديمية
Rif1 restrains the rate of replication origin firing in Xenopus laevis
| العنوان: | Rif1 restrains the rate of replication origin firing in Xenopus laevis |
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| المؤلفون: | Haccard, Olivier, Ciardo, Diletta, Narrissamprakash, Hemalatha, Bronchain, Odile, Kumagai, Akiko, Dunphy, William, G, Goldar, Arach, Marheineke, Kathrin |
| المساهمون: | Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut de biologie de l'ENS Paris (IBENS), Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS-PSL), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut des Neurosciences Paris-Saclay (NeuroPSI), Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Division of Biology and Biological Engineering Pasadena, USA (BBE), California Institute of Technology (CALTECH), ARC (Association pour la Recherche sur le Cancer)Association Retina France, Fondation Valentin Haüy, and UNADEV (Union Nationale des Aveugles et Déficients Visuels)ITMO NNP (Institut Thématique Multi-Organisme Neurosciences, sciences cognitives, neurologie, psychiatrie)/AVIESAN (alliance nationale pour les sciences de la vie et de la santé).IDEX Paris-Saclay University Ph.D.National Institutes of Health grant R01 GM043974. |
| المصدر: | ISSN: 2399-3642 ; Communications Biology ; https://hal.science/hal-04178050Test ; Communications Biology, 2023, 6 (1), pp.788. ⟨10.1038/s42003-023-05172-8⟩. |
| بيانات النشر: | HAL CCSD Nature Publishing Group |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Rif1, DNA replication, Timing program, MTBP, Treslin, DDK, Xenopus laevis, early embryonic development, MESH: Animals, Cell Cycle, Cell Cycle Proteins* / genetics, Chromatin / genetics, Replication Origin, Xenopus laevis / genetics, [SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology |
| الوصف: | International audience ; Metazoan genomes are duplicated by the coordinated activation of clusters of replication origins at different times during S phase, but the underlying mechanisms of this temporal program remain unclear during early development. Rif1, a key replication timing factor, inhibits origin firing by recruiting protein phosphatase 1 (PP1) to chromatin counteracting S phase kinases. We have previously described that Rif1 depletion accelerates early Xenopus laevis embryonic cell cycles. Here, we find that in the absence of Rif1, patterns of replication foci change along with the acceleration of replication cluster activation. However, initiations increase only moderately inside active clusters. Our numerical simulations suggest that the absence of Rif1 compresses the temporal program towards more homogeneity and increases the availability of limiting initiation factors. We experimentally demonstrate that Rif1 depletion increases the chromatin-binding of the S phase kinase Cdc7/Drf1, the firing factors Treslin, MTBP, Cdc45, RecQL4, and the phosphorylation of both Treslin and MTBP. We show that Rif1 globally, but not locally, restrains the replication program in early embryos, possibly by inhibiting or excluding replication factors from chromatin. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/37516798; hal-04178050; https://hal.science/hal-04178050Test; https://hal.science/hal-04178050/documentTest; https://hal.science/hal-04178050/file/s42003-023-05172-8.pdfTest; PUBMED: 37516798; PUBMEDCENTRAL: PMC10387115 |
| DOI: | 10.1038/s42003-023-05172-8 |
| الإتاحة: | https://doi.org/10.1038/s42003-023-05172-8Test https://hal.science/hal-04178050Test https://hal.science/hal-04178050/documentTest https://hal.science/hal-04178050/file/s42003-023-05172-8.pdfTest |
| حقوق: | http://creativecommons.org/licenses/byTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.A99070C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s42003-023-05172-8 |
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