دورية أكاديمية
Uterine secretome and its modulation in rat (Rattus norvegicus)
| العنوان: | Uterine secretome and its modulation in rat (Rattus norvegicus) |
|---|---|
| المؤلفون: | Bhutada, Sumit, Katkam, R R, Nandedkar, Tarla, Metkari, S M, Chaudhari, U K, Varghese, Sneha, Kholkute, S D, Sachdeva, Geetanjali |
| المصدر: | REPRODUCTION ; volume 146, issue 1, page 13-26 ; ISSN 1470-1626 1741-7899 |
| بيانات النشر: | Bioscientifica |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Cell Biology, Obstetrics and Gynecology, Endocrinology, Embryology, Reproductive Medicine |
| الوصف: | The present study identifies uterine fluid (UF) proteins that display differential abundance during the embryo-permissive phase in nonconception and conception cycles in rats. UF samples were collected from nonpregnant rats in the proestrous ( n =17) and metestrous ( n =18) phases and also from pregnant ( n =17) and pseudopregnant ( n =17) rats on day 4 post coitus. UF protein profile in the metestrous phase was compared with that in the proestrous phase. Similarly, UF protein profile of the pregnant rats was compared with that of the pseudopregnant rats. Two-dimensional PAGE, followed by densitometric analysis of the paired protein spots, revealed differential abundance of 44 proteins in the metestrous phase, compared with that in the proestrous phase. Of these, 29 proteins were identified by matrix-assisted laser desorption/ionization time-of-flight or liquid chromatography–tandem mass spectrometry. Functional groups such as proteases, protease inhibitors, and oxidoreductases were enriched in differentially abundant proteins. Total protease activity in UF was found to be significantly ( P <0.05; t -test) higher in the proestrous phase, compared with that in the metestrous phase. Furthermore, 41 UF proteins were found to be differentially abundant in pregnant rats, compared with pseudopregnant rats. Of these, 11 proteins could be identified. Immunoblotting analysis confirmed significantly higher ( P <0.05; t -test) abundance of β-actin, Rho-specific guanine nucleotide dissociation inhibitor alpha (Rho-GDIα), and peroxiredoxin-2 and -6 in the metestrous phase, compared with that in the proestrous phase. Compared with pseudopregnant rats, pregnant rats had significantly higher ( P <0.05; t -test) levels of UF β-actin and Rho-GDIα. Furthermore, these proteins could be detected in the culture supernatants of endometrial epithelial cell lines, thereby providing an evidence of their secretion from endometrial epithelial cells. Data obtained from the study expand our knowledge on the uterine milieu that ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| DOI: | 10.1530/rep-12-0461 |
| الإتاحة: | https://doi.org/10.1530/rep-12-0461Test https://rep.bioscientifica.com/view/journals/rep/146/1/13.xmlTest https://rep.bioscientifica.com/downloadpdf/journals/rep/146/1/13.xmlTest |
| رقم الانضمام: | edsbas.5FCC7E34 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1530/rep-12-0461 |
|---|