التفاصيل البيبلوغرافية
| العنوان: |
NO impact on cell energy and LIP, 1 IMPACT OF ENDOGENOUS NITRIC OXIDE ON MICROGLIAL CELL ENERGY METABOLISM AND LABILE IRON POOL. |
| المؤلفون: |
Benoît Chénais, Hamid Morjani, Jean-claude Drapier |
| المساهمون: |
The Pennsylvania State University CiteSeerX Archives |
| المصدر: |
http://hal.archives-ouvertes.fr/docs/00/42/29/11/PDF/JNC02.PDFTest. |
| سنة النشر: |
2009 |
| المجموعة: |
CiteSeerX |
| مصطلحات موضوعية: |
electron transport chain, FA, Friedreich’s ataxia, FITC, fluoresceine-isothiocyanate, IFN-γ, γ-interferon, IRE, iron responsive element, IRP, iron regulatory protein, LDH, lactate dehydrogenase, LIP, labile iron pool, L-NIL, N 6-(1-imminoethyl)-L-lysine, L-NMMA, N- monomethyl-L-arginine, LPS, lipopolysaccharide, NO, nitric oxide, NOS, nitric oxide synthase, PBS, phosphate-buffered saline, PD, Parkinson’s disease, ROS |
| الوصف: |
untranslated region. NO impact on cell energy and LIP, 2 Abstract: Microglial activation is common in several neurodegenerative disorders. In the present study, we used the murine BV-2 microglial cell line stimulated with γ-interferon and lipopolysaccharide to gain new insights into the effects of endogenously produced NO on mitochondrial respiratory capacity, iron regulatory protein activity, and redox-active iron level. Using polarographic measurement of respiration of both intact and digitoninpermeabilized cells, and spectrophotometric determination of individual respiratory chain complex activity, we showed that in addition to the reversible inhibition of cytochrome-c oxidase, long-term endogenous NO production reduced complex I and complex II hal-00422911, version 1- 8 Oct 2009 activities in an irreversible manner. As a consequence, the cellular ATP level was decreased in NO-producing cells, whereas ATPase activity was unaffected. We show that |
| نوع الوثيقة: |
text |
| اللغة: |
English |
| العلاقة: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.322.8466Test; http://hal.archives-ouvertes.fr/docs/00/42/29/11/PDF/JNC02.PDFTest |
| الإتاحة: |
http://hal.archives-ouvertes.fr/docs/00/42/29/11/PDF/JNC02.PDFTest |
| حقوق: |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: |
edsbas.8618DDA7 |
| قاعدة البيانات: |
BASE |
