دورية أكاديمية
Single-cell RNA sequencing identifies shared differentiation paths of mouse thymic innate T cells
| العنوان: | Single-cell RNA sequencing identifies shared differentiation paths of mouse thymic innate T cells |
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| المساهمون: | Minji Lee, Eunmin Lee, Seong Kyu Han, Yoon Ha Choi, Dong-Il Kwon, Hyobeen Choi, Kwanghwan Lee, Eun Seo Park, Min-Seok Rha, Dong Jin Joo, Eui-Cheol Shin, Sanguk Kim, Jong Kyoung Kim, You Jeong Lee, Joo, Dong Jin |
| بيانات النشر: | Nature Pub. Group |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Animals, Cell Differentiation, Cells, Cultured, Clonal Selection, Antigen-Mediated, Humans, Immunity, Innate, Liver / cytology, Liver / immunology, Lymphocyte Activation, Mice, Mucosal-Associated Invariant T Cells / metabolism, Natural Killer T-Cells / metabolism, Sequence Analysis, RNA, Single-Cell Analysis, T-Lymphocytes / metabolism, Th17 Cells / metabolism, Thymus Gland / cytology, Thymus Gland / immunology |
| الوصف: | Invariant natural killer T (iNKT), mucosal-associated invariant T (MAIT), and γδ T cells are innate T cells that acquire memory phenotype in the thymus and share similar biological characteristics. However, how their effector differentiation is developmentally regulated is still unclear. Here, we identify analogous effector subsets of these three innate T cell types in the thymus that share transcriptional profiles. Using single-cell RNA sequencing, we show that iNKT, MAIT and γδ T cells mature via shared, branched differentiation rather than linear maturation or TCR-mediated instruction. Simultaneous TCR clonotyping analysis reveals that thymic maturation of all three types is accompanied by clonal selection and expansion. Analyses of mice deficient of TBET, GATA3 or RORγt and additional in vivo experiments corroborate the predicted differentiation paths, while human innate T cells from liver samples display similar features. Collectively, our data indicate that innate T cells share effector differentiation processes in the thymus. ; open |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2041-1723 |
| العلاقة: | NATURE COMMUNICATIONS; J02293; OAK-2021-02655; https://ir.ymlib.yonsei.ac.kr/handle/22282913/183949Test; T202007265; NATURE COMMUNICATIONS, Vol.11(1) : 4367, 2020-08 |
| DOI: | 10.1038/s41467-020-18155-8 |
| الإتاحة: | https://doi.org/10.1038/s41467-020-18155-8Test https://ir.ymlib.yonsei.ac.kr/handle/22282913/183949Test |
| حقوق: | CC BY-NC-ND 2.0 KR |
| رقم الانضمام: | edsbas.C8318F28 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20411723 |
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| DOI: | 10.1038/s41467-020-18155-8 |