MiR-137 inhibits cervical cancer progression via down-modulating Notch1 and inhibiting the PI3K/AKT/mTOR signaling pathway
| العنوان: | MiR-137 inhibits cervical cancer progression via down-modulating Notch1 and inhibiting the PI3K/AKT/mTOR signaling pathway |
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| المؤلفون: | Lina Wang, Zhihong Huang, Ying Gui |
| المصدر: | Translational Cancer Research |
| بيانات النشر: | AME Publishing Company, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | cervical cancer (CC), Cervical cancer, Notch1, Cancer Research, business.industry, proliferation, MTOR signaling pathway, medicine.disease, mir-137, MicroRNA-137 (miR-137), Oncology, Cancer research, metastasis, Medicine, Original Article, Radiology, Nuclear Medicine and imaging, business, Protein kinase B, PI3K/AKT/mTOR pathway |
| الوصف: | Background Cervical cancer (CC) is frequently diagnosed, and accounts for a large proportion of cancer-related mortality worldwide. Therefore, it is an urgent need to explore the mechanisms of CC progression and seek new therapeutic targets. We know that microRNA-137 (miR-137) is involved in CC progression; however, it’s mechanism in CC has not yet been clarified. Methods Quantitative real-time PCR (qRT-PCR) was applied to detect the expressions of miR-137 and Notch homolog 1 messenger RNA (Notch1 mRNA) in CC cells. Cell Counting Kit-8 (CCK-8) and Transwell assay were utilized to monitor the proliferation, invasion, and migration of CC cells. The binding relationship of miR-137 with Notch1 was detected by dual luciferase reporter gene assay. Western blot was used to determine the expressions of Notch1, p-AKT/AKT, and p-mTOR/mTOR signaling pathways. Results The results showed that miR-137 was down-regulated and Notch1 was up-regulated in CC cells. MiR-137 was reversely correlated with Notch1, and Notch1 partially reversed the promotion of miR-137 silencing on malignant phenotype of CC cells. Furthermore, in vitro experiments confirmed that miR-137 partially impeded CC progression via regulation of the Notch1 and PI3K/AKT/mTOR axis. Conclusions MiR-137 targets Notch1 and regulates CC through the PI3K/Akt/mTOR pathway. The miR-137/Notch1 axis may be a promising target for CC patients, and these findings can provide a reference for the clinical treatment of CC. |
| تدمد: | 2219-6803 2218-676X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c5578c8f3319bd27bc07e569a727aa78Test https://doi.org/10.21037/tcr-21-1049Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c5578c8f3319bd27bc07e569a727aa78 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22196803 2218676X |
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