دورية أكاديمية
RSV and HMPV infections in 3d tissue cultures: mechanisms involved in virus-host and virus-virus interactions
| العنوان: | RSV and HMPV infections in 3d tissue cultures: mechanisms involved in virus-host and virus-virus interactions |
|---|---|
| المؤلفون: | Geiser, Johan, Boivin, Guy, Huang, Song, Constant, Samuel, Kaiser, Laurent, Tapparel, Caroline, Essaidi, Manel |
| المصدر: | ISSN: 1999-4915 ; Viruses, vol. 13, no. 1 (2021) 139. |
| سنة النشر: | 2021 |
| المجموعة: | Université de Genève: Archive ouverte UNIGE |
| مصطلحات موضوعية: | info:eu-repo/classification/ddc/616, Cell Culture Techniques, Cell Line, Coinfection, Host-Pathogen Interactions, Humans, Interferon Type I/pharmacology, Interferons/pharmacology, Metapneumovirus/drug effects/physiology, Microbial Interactions, Paramyxoviridae Infections/virology, Respiratory Syncytial Virus Infections/virology, Respiratory Syncytial Virus, Human/drug effects/physiology, Spheroids, Cellular, Virus Replication |
| الوصف: | Respiratory viral infections constitute a global public health concern. Among prevalent respiratory viruses, two pneumoviruses can be life-threatening in high-risk populations. In young children, they constitute the first cause of hospitalization due to severe lower respiratory tract diseases. A better understanding of their pathogenesis is still needed as there are no approved efficient anti-viral nor vaccine against pneumoviruses. We studied Respiratory Syncytial virus (RSV) and human Metapneumovirus (HMPV) in single and dual infections in three-dimensional cultures, a highly relevant model to study viral respiratory infections of the airway epithelium. Our investigation showed that HMPV is less pathogenic than RSV in this model. Compared to RSV, HMPV replicated less efficiently, induced a lower immune response, did not block cilia beating, and was more sensitive to IFNs. In dual infections, RSV-infected epithelia were less permissive to HMPV. By neutralizing IFNs in co-infection assays, we partially prevented HMPV inhibition by RSV and significantly increased the number of co-infected cells in the tissue. This suggests that interference in dual infection would be at least partly mediated by the host immune response. In summary, this work provides new insight regarding virus-host and virus-virus interactions of pneumoviruses in the airway epithelium. This could be helpful for the proper handling of at-risk patients. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/33478119; https://archive-ouverte.unige.ch/unige:151851Test; unige:151851 |
| الإتاحة: | https://doi.org/10.3390/v13010139Test https://archive-ouverte.unige.ch/unige:151851Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.BE1F58E7 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |