دورية أكاديمية
G protein‐coupled receptors can control the Hippo/YAP pathway through Gq signaling
| العنوان: | G protein‐coupled receptors can control the Hippo/YAP pathway through Gq signaling |
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| المؤلفون: | Zindel, Diana, Mensat, Patrick, Vol, Claire, Homayed, Zeinab, Charrier‐savournin, Fabienne, Trinquet, Eric, Banères, Jean‐louis, Pin, Jean-Philippe, Pannequin, Julie, Roux, Thomas, Dupuis, Elodie, Prézeau, Laurent |
| المساهمون: | Institut de Génomique Fonctionnelle (IGF), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Cisbio Bioassays, Institut des Biomolécules Max Mousseron Pôle Chimie Balard (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | ISSN: 0892-6638. |
| بيانات النشر: | HAL CCSD Federation of American Society of Experimental Biology |
| سنة النشر: | 2021 |
| المجموعة: | Inserm: HAL (Institut national de la santé et de la recherche médicale) |
| مصطلحات موضوعية: | inverse agonist, constitutive activity, cellular signaling, Hippo pathway, Ghrelin receptor, MESH: Activating Transcription Factor 6, MESH: Cell Cycle Proteins, MESH: Receptors, G-Protein-Coupled, MESH: Transcription Factors, MESH: GTP-Binding Protein alpha Subunits, G12-G13, Gq-G11, MESH: Gene Expression Regulation, MESH: HEK293 Cells, MESH: Humans, MESH: Phosphorylation, MESH: Protein-Serine-Threonine Kinases, [SDV]Life Sciences [q-bio], [SDV.BC]Life Sciences [q-bio]/Cellular Biology |
| الوصف: | International audience ; The Hippo pathway is an evolutionarily conserved kinase cascade involved in the control of tissue homeostasis, cellular differentiation, proliferation, and organ size, and is regulated by cell-cell contact, apical cell polarity, and mechanical signals. Miss-regulation of this pathway can lead to cancer. The Hippo pathway acts through the inhibition of the transcriptional coactivators YAP and TAZ through phosphorylation. Among the various signaling mechanisms controlling the hippo pathway, activation of G12/13 by G protein-coupled receptors (GPCR) recently emerged. Here we show that a GPCR, the ghrelin receptor, that activates several types of G proteins, including G12/13, Gi/o, and Gq, can activate YAP through Gq/11 exclusively, independently of G12/13. We revealed that a strong basal YAP activation results from the high constitutive activity of this receptor, which can be further increased upon agonist activation. Thus, acting on ghrelin receptor allowed to modulate up-and-down YAP activity, as activating the receptor increased YAP activity and blocking constitutive activity reduced YAP activity. Our results demonstrate that GPCRs can be used as molecular switches to finely up- or down-regulate YAP activity through a pure Gq pathway. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/34114695; hal-03263933; https://hal.science/hal-03263933Test; https://hal.science/hal-03263933/documentTest; https://hal.science/hal-03263933/file/211118-Zindel-FASEB%20J-2021-HAL%20%281%29.pdfTest; PUBMED: 34114695 |
| DOI: | 10.1096/fj.202002159R |
| الإتاحة: | https://doi.org/10.1096/fj.202002159RTest https://hal.science/hal-03263933Test https://hal.science/hal-03263933/documentTest https://hal.science/hal-03263933/file/211118-Zindel-FASEB%20J-2021-HAL%20%281%29.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.AE495F8C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1096/fj.202002159R |
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