دورية أكاديمية
Snapshot study of canine distemper virus in Bangladesh with on-site PCR detection and nanopore sequencing
| العنوان: | Snapshot study of canine distemper virus in Bangladesh with on-site PCR detection and nanopore sequencing |
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| المؤلفون: | Zsófia Lanszki, Md. Shafeul Islam, Md. Foisal Shikder, Md. Jalal Uddin Sarder, Shahneaz Ali Khan, Sharmin Chowdhury, Md. Nurul Islam, Zsófia Tauber, Gábor Endre Tóth, Ferenc Jakab, Gábor Kemenesi, Sazeda Akter |
| المصدر: | Scientific Reports, Vol 14, Iss 1, Pp 1-7 (2024) |
| بيانات النشر: | Nature Portfolio, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | Abstract Canine distemper virus (CDV) is a highly contagious virus that affects domestic and wild animals, causing severe illness with high mortality rates. Rapid monitoring and sequencing can provide valuable information about circulating CDV strains, which may foster effective vaccination strategies and the successful integration of these into conservation programs. During two site visits in Bangladesh in 2023, we tested a mobile, deployable genomic surveillance setup to explore the genetic diversity and phylogenetic patterns of locally circulating CDV strains. We collected and analysed 355 oral swab samples from stray dogs in Rajshahi and Chattogram cities, Bangladesh. CDV-specific real-time RT-PCR was performed to screen the samples. Out of the 355 samples, 7.4% (10/135) from Rajshahi city and 0.9% (2/220) from Chattogram city tested positive for CDV. We applied a real-time RT-PCR assay and a pan-genotype CDV-specific amplicon-based Nanopore sequencing technology to obtain the near-completes. Five near-complete genome sequences were generated, with phylogenetic relation to the India-1/Asia-5 lineage previously identified in India. This is the first study to provide genomic data on CDV in Bangladesh and the first demonstration of a mobile laboratory setup as a powerful tool in rapid genomic surveillance and risk assessment for CDV in low resource regions. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2045-2322 |
| العلاقة: | https://doaj.org/toc/2045-2322Test |
| DOI: | 10.1038/s41598-024-59343-6 |
| الوصول الحر: | https://doaj.org/article/52a287857a9241b1a91ccc992f4b3cdeTest |
| رقم الانضمام: | edsdoj.52a287857a9241b1a91ccc992f4b3cde |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20452322 |
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| DOI: | 10.1038/s41598-024-59343-6 |