دورية أكاديمية
Sputum microbiome and chronic obstructive pulmonary disease in a rural Ugandan cohort of well-controlled HIV infection
| العنوان: | Sputum microbiome and chronic obstructive pulmonary disease in a rural Ugandan cohort of well-controlled HIV infection |
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| المؤلفون: | Kayongo, A., Bartolomaeus, T.U.P., Birkner, T., Markó, L., Löber, U., Kigozi, E., Atugonza, C., Munana, R., Mawanda, D., Sekibira, R., Uwimaana, E., Alupo, P., Kalyesubula, R., Knauf, F., Siddharthan, T., Bagaya, B.S., Kateete, D.P., Joloba, M.L., Sewankambo, N.K., Jjingo, D., Kirenga, B., Checkley, W., Forslund, S.K. |
| بيانات النشر: | American Society for Microbiology |
| سنة النشر: | 2023 |
| المجموعة: | Max-Delbrueck-Center for Molecular Medicine, Berlin: MDC Repository |
| مصطلحات موضوعية: | Cardiovascular and Metabolic Diseases, Topic 3: Integrative Biomedicine |
| الوصف: | Sub-Saharan Africa has increased morbidity and mortality related to chronic obstructive pulmonary disease (COPD). COPD among people living with HIV (PLWH) has not been well studied in this region, where HIV/AIDS is endemic. Increasing evidence suggests that respiratory microbial composition plays a role in COPD severity. Therefore, we aimed to investigate microbiome patterns and associations among PLWH with COPD in Sub-Saharan Africa. We conducted a cross-sectional study of 200 adults stratified by HIV and COPD in rural Uganda. Induced sputum samples were collected as an easy-to-obtain proxy for the lower respiratory tract microbiota. We performed 16S rRNA gene sequencing and used PICRUSt2 (version 2.2.3) to infer the functional profiles of the microbial community. We used a statistical tool to detect changes in specific taxa that searches and adjusts for confounding factors such as antiretroviral therapy (ART), age, sex, and other participant characteristics. We could cluster the microbial community into three community types whose distribution was shown to be significantly impacted by HIV. Some genera, e.g., Veillonella, Actinomyces, Atopobium, and Filifactor, were significantly enriched in HIV-infected individuals, while the COPD status was significantly associated with Gammaproteobacteria and Selenomonas abundance. Furthermore, reduced bacterial richness and significant enrichment in Campylobacter were associated with HIV-COPD comorbidity. Functional prediction using PICRUSt2 revealed a significant depletion in glutamate degradation capacity pathways in HIV-positive patients. A comparison of our findings with an HIV cohort from the United Kingdom revealed significant differences in the sputum microbiome composition, irrespective of viral suppression. IMPORTANCE: Even with ART available, HIV-infected individuals are at high risk of suffering comorbidities, as shown by the high prevalence of noninfectious lung diseases in the HIV population. Recent studies have suggested a role for the respiratory microbiota ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | http://edoc.mdc-berlin.de/23146/1/23146oa.pdfTest; http://edoc.mdc-berlin.de/23146/7/23146suppl.pdfTest; Sputum microbiome and chronic obstructive pulmonary disease in a rural Ugandan cohort of well-controlled HIV infection. Kayongo, A. and Bartolomaeus, T.U.P. and Birkner, T. and Markó, L. and Löber, U. and Kigozi, E. and Atugonza, C. and Munana, R. and Mawanda, D. and Sekibira, R. and Uwimaana, E. and Alupo, P. and Kalyesubula, R. and Knauf, F. and Siddharthan, T. and Bagaya, B.S. and Kateete, D.P. and Joloba, M.L. and Sewankambo, N.K. and Jjingo, D. and Kirenga, B. and Checkley, W. and Forslund, S.K. Microbiology Spectrum : e0213921. 15 February 2023 (In Press) |
| DOI: | 10.1128/spectrum.02139-21 |
| الإتاحة: | https://doi.org/10.1128/spectrum.02139-21Test http://edoc.mdc-berlin.de/23146Test/ https://edoc.mdc-berlin.de/23146Test/ http://edoc.mdc-berlin.de/23146/1/23146oa.pdfTest http://edoc.mdc-berlin.de/23146/7/23146suppl.pdfTest |
| حقوق: | cc_by_4 |
| رقم الانضمام: | edsbas.809946EE |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/spectrum.02139-21 |
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