التفاصيل البيبلوغرافية
| العنوان: |
Nanoparticle-Enhanced Surface Plasmon Resonance Imaging Enables the Ultrasensitive Detection of Non-Amplified Cell-Free Fetal DNA for Non-Invasive Prenatal Testing |
| المؤلفون: |
Marzia Calcagno (11885891), Roberta D’Agata (2147104), Giulia Breveglieri (717941), Monica Borgatti (375543), Noemi Bellassai (10872809), Roberto Gambari (328450), Giuseppe Spoto (1373259) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Medicine, Cell Biology, Molecular Biology, Biotechnology, Cancer, Chemical Sciences not elsewhere classified, sample contamination pcr, invasive prenatal testing, female genomic dnas, early clinical diagnosis, different gestational ages, chromosome specific sequence, approach would pave, free fetal dna, dna target sequence, maternal blood analysis, spr assay proposed, enhanced spri strategy, reveal male cell, male dna, thus establishing, successfully applied, require costly, preliminary amplification, pregnant women, maternal plasma, gene sry, developed protocol, based procedures |
| الوصف: |
Although many potential applications in early clinical diagnosis have been proposed, the use of a surface plasmon resonance imaging (SPRI) technique for non-invasive prenatal diagnostic approaches based on maternal blood analysis is confined. Here, we report a nanoparticle-enhanced SPRI strategy for a non-invasive prenatal fetal sex determination based on the detection of a Y-chromosome specific sequence (single-gene SRY) in cell-free fetal DNA from maternal plasma. The SPR assay proposed here allows for detection of male DNA in mixtures of 2.5 aM male and female genomic DNAs with no preliminary amplification of the DNA target sequence, thus establishing an analytical protocol that does not require costly, time-consuming, and prone to sample contamination PCR-based procedures. Afterward, the developed protocol was successfully applied to reveal male cell-free fetal DNA in the plasma of pregnant women at different gestational ages, including early gestational ages. This approach would pave the way for the establishment of faster and cost-effective non-invasive prenatal testing. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/journal_contribution/Nanoparticle-Enhanced_Surface_Plasmon_Resonance_Imaging_Enables_the_Ultrasensitive_Detection_of_Non-Amplified_Cell-Free_Fetal_DNA_for_Non-Invasive_Prenatal_Testing/17702852Test |
| DOI: |
10.1021/acs.analchem.1c04196.s001 |
| الإتاحة: |
https://doi.org/10.1021/acs.analchem.1c04196.s001Test |
| حقوق: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.43D1802A |
| قاعدة البيانات: |
BASE |
