دورية أكاديمية
DNA sequencing in the classroom: complete genome sequence of two earwig (Dermaptera; Insecta) species
| العنوان: | DNA sequencing in the classroom: complete genome sequence of two earwig (Dermaptera; Insecta) species |
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| المؤلفون: | Kobayashi, Sanae, Maldonado, Jonathan E., Gaete, Alexis, Araya, Ingrid, Aguado-Norese, Constanza, Cumplido, Nicolás, Díaz, Sebastián, Espinoza, Alonso, Fernández, Edelmira, Gajardo, Felipe, González-Ordenes, Felipe, Hauyon, Khantati, Maldonado, Piedad, Maldonado, Rodrigo, Pochet, Isabel, Riveros, Aníbal, Sandoval, Paula, Sepúlveda-González, Ailynne, Stuardo, Camila, Tapia-Reyes, Patricio, Thornton, Carolina, Undurraga, Soledad, Varas, Macarena, Valdivieso, Camilo, Gutiérrez Ilabaca, Rodrigo Antonio, Orellana, Ariel, Montecino, Martín, Maass, Alejandro, González, Mauricio, Allende, Miguel L., Hodar, Christian, Irles, Paula |
| سنة النشر: | 2023 |
| المجموعة: | Pontificia Universidad Católica de Chile: Repositorio UC |
| مصطلحات موضوعية: | Euborellia annulipes, Forficula auricularia, Nanopore sequencing, Citizen Science, Biología |
| الوقت: | 570 |
| الوصف: | Background Despite representing the largest fraction of animal life, the number of insect species whose genome has been sequenced is barely in the hundreds. The order Dermaptera (the earwigs) suffers from a lack of genomic information despite its unique position as one of the basally derived insect groups and its importance in agroecosystems. As part of a national educational and outreach program in genomics, a plan was formulated to engage the participation of high school students in a genome sequencing project. Students from twelve schools across Chile were instructed to capture earwig specimens in their geographical area, to identify them and to provide material for genome sequencing to be carried out by themselves in their schools. Results The school students collected specimens from two cosmopolitan earwig species: Euborellia annulipes (Fam. Anisolabididae) and Forficula auricularia (Fam. Forficulidae). Genomic DNA was extracted and, with the help of scientific teams that traveled to the schools, was sequenced using nanopore sequencers. The sequence data obtained for both species was assembled and annotated. We obtained genome sizes of 1.18 Gb (F. auricularia) and 0.94 Gb (E. annulipes) with the number of predicted protein coding genes being 31,800 and 40,000, respectively. Our analysis showed that we were able to capture a high percentage (≥ 93%) of conserved proteins indicating genomes that are useful for comparative and functional analysis. We were also able to characterize structural elements such as repetitive sequences and non-coding RNA genes. Finally, functional categories of genes that are overrepresented in each species suggest important differences in the process underlying the formation of germ cells, and modes of reproduction between them, features that are one of the distinguishing biological properties that characterize these two distant families of Dermaptera. Conclusions This work represents an unprecedented instance where the scientific and lay community have come together to collaborate ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0717-6287 |
| العلاقة: | Kobayashi, S., Maldonado, J.E., Gaete, A. et al. DNA sequencing in the classroom: complete genome sequence of two earwig (Dermaptera; Insecta) species. Biol Res 56, 6 (2023). https://doi.org/10.1186/s40659-023-00414-9Test; https://doi.org/10.1186/s40659-023-00414-9Test; https://repositorio.uc.cl/handle/11534/66648Test |
| DOI: | 10.1186/s40659-023-00414-9 |
| الإتاحة: | https://doi.org/10.1186/s40659-023-00414-9Test https://repositorio.uc.cl/handle/11534/66648Test |
| حقوق: | acceso abierto ; https://creativecommons.org/licenses/by/4.0Test/ ; The Author(s) ; Attribution 4.0 International (CC BY 4.0) |
| رقم الانضمام: | edsbas.BFB98CE |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 07176287 |
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| DOI: | 10.1186/s40659-023-00414-9 |