التفاصيل البيبلوغرافية
| العنوان: |
Endocrine activities and adipogenic effects of bisphenol AF and its main metabolite |
| المؤلفون: |
Skledar, Darja Gramec, Carino, Adriana, Trontelj, Jurij, Troberg, Johanna, Distrutti, Eleonora, Marchiano, Silvia, Tornasic, Tihomir, Zega, Anamarija, Finel, Moshe, Fiorucci, Stefano, Maisic, Lucija Peterlin |
| المساهمون: |
Faculty of Pharmacy, Division of Pharmaceutical Chemistry and Technology, Pharmaceutical Design and Discovery group, Drug Research Program, Moshe Finel / Principal Investigator |
| بيانات النشر: |
Elsevier Scientific Publ. Co |
| سنة النشر: |
2021 |
| المجموعة: |
Helsingfors Universitet: HELDA – Helsingin yliopiston digitaalinen arkisto |
| مصطلحات موضوعية: |
Bisphenol AF, Bisphenol AF glucuronide, Glucuronidation, Endocrine activities, Lipid accumulation, IN-VITRO, SEWAGE-SLUDGE, ANALOGS, CELLS, TOXICITY, GAMMA, IDENTIFICATION, PREADIPOCYTES, ANTAGONIST, Biochemistry, cell and molecular biology |
| الوصف: |
Bisphenol AF (BPAF) is a fluorinated analog of bisphenol A (BPA), and it is a more potent estrogen receptor (ER) agonist. BPAF is mainly metabolized to BPAF-glucuronide (BPAF-G), which has been reported to lack ER agonist activity and is believed to be biologically inactive. The main goal of the current study was to examine the influence of the metabolism of BPAF via glucuronidation on its ER activity and adipogenesis. Also, as metabolites can have different biological activities, the effects of BPAF-G on other nuclear receptors were evaluated. First, in-vitro BPAF glucuronidation was investigated using recombinant human enzymes. Specific reporter-gene assays were used to determine BPAF and BPAF-G effects on estrogen, androgen, glucocorticoid, and thyroid receptor pathways, and on PXR, FXR, and PPAR gamma pathways. Their effects on lipid accumulation and differentiation were determined in murine 3T3L1 preadipocytes using Nile Red, with mRNA expression analysis of the adipogenic markers adiponectin, Fabp4, Cebp alpha, and PPAR gamma. BPAF showed strong agonistic activity for hER alpha and moderate antagonistic activities for androgen and thyroid receptors, and for PXR. BPAF-G was antagonistic for PXR and PPAR gamma. BPAF (0.1 mu M) and BPAF-G (1.0 mu M) induced lipid accumulation and increased expression of key adipogenic markers in murine preadipocytes. BPAF-G is therefore not an inactive metabolite of BPAF. Further toxicological and epidemiological investigations of BPAF effects on human health are warranted, to provide better understanding of the metabolic end-elimination of BPAF. (C) 2018 Elsevier Ltd. All rights reserved. ; Peer reviewed |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
The authors thank OpenEye Scientific Software, Santa Fe, NM, USA, for free academic licenses for the use of their software, and Johanna Mosorin for skillful assistance in recombinant UGT preparations. Financial support of the Slovenian Research Agency (Grant No. P1-0208) and the Sigrid Juseliuksen Saatio, Finland (grant no. 4704583) are acknowledged.; Skledar , D G , Carino , A , Trontelj , J , Troberg , J , Distrutti , E , Marchiano , S , Tornasic , T , Zega , A , Finel , M , Fiorucci , S & Maisic , L P 2019 , ' Endocrine activities and adipogenic effects of bisphenol AF and its main metabolite ' , Chemosphere , vol. 215 , pp. 870-880 . https://doi.org/10.1016/j.chemosphere.2018.10.129Test; ORCID: /0000-0003-3886-4771/work/51564915; http://hdl.handle.net/10138/325049Test; f063b300-8d14-4568-8375-b75ca2da9adc; 85055983814; 000450383400096 |
| الإتاحة: |
http://hdl.handle.net/10138/325049Test |
| حقوق: |
cc_by_nc_nd ; info:eu-repo/semantics/openAccess ; openAccess |
| رقم الانضمام: |
edsbas.BCD201F1 |
| قاعدة البيانات: |
BASE |
