Impaired acetylcholine-induced cutaneous vasodilation in young smokers: roles of nitric oxide and prostanoids
| العنوان: | Impaired acetylcholine-induced cutaneous vasodilation in young smokers: roles of nitric oxide and prostanoids |
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| المؤلفون: | Christopher T. Minson, Maggie Cooper Reinke, Naoto Fujii, Vienna E. Brunt |
| المصدر: | American Journal of Physiology-Heart and Circulatory Physiology. 304:H667-H673 |
| بيانات النشر: | American Physiological Society, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Male, medicine.medical_specialty, Mean arterial pressure, Microdialysis, Physiology, Vascular Biology and Microcirculation, Vasodilator Agents, Vasodilation, Nitric Oxide, Nitric oxide, Young Adult, chemistry.chemical_compound, Physiology (medical), Internal medicine, Laser-Doppler Flowmetry, medicine, Humans, Cyclooxygenase Inhibitors, Enzyme Inhibitors, Skin, biology, business.industry, Microcirculation, Smoking, Area under the curve, equipment and supplies, Acetylcholine, Skin Aging, Ketorolac, NG-Nitroarginine Methyl Ester, Endocrinology, chemistry, Prostaglandin-Endoperoxide Synthases, Anesthesia, Prostaglandins, biology.protein, Female, Sodium nitroprusside, Cyclooxygenase, Cardiology and Cardiovascular Medicine, business, medicine.drug |
| الوصف: | Cigarette smoking attenuates acetylcholine (ACh)-induced cutaneous vasodilation in humans, but the underlying mechanisms are unknown. We tested the hypothesis that smokers have impaired nitric oxide (NO)- and cyclooxygenase (COX)-dependent cutaneous vasodilation to ACh infusion. Twelve young smokers, who have smoked more than 5.2 ± 0.7 yr with an average daily consumption of 11.4 ± 1.2 cigarettes, and 12 nonsmokers were tested. Age, body mass index, and resting mean arterial pressure were similar between the groups. Cutaneous vascular conductance (CVC) was evaluated as laser-Doppler flux divided by mean arterial pressure, normalized to maximal CVC (local heating to 43.0°C plus sodium nitroprusside administration). We evaluated the increase in CVC from baseline to peak (CVCΔpeak) and area under the curve of CVC (CVCAUC) during a bolus infusion (1 min) of 137.5 μM ACh at four intradermal microdialysis sites: 1) Ringer (control), 2) 10 mM NG-nitro-l-arginine methyl ester (l-NAME; NO synthase inhibitor), 3) 10 mM ketorolac (COX inhibitor), and 4) combination of l-NAME + ketorolac. CVCΔpeakand CVCAUCat the Ringer site in nonsmokers were greater than in smokers (CVCΔpeak, 42.9 ± 5.1 vs. 22.3 ± 3.5%max, P < 0.05; and CVCAUC, 8,085 ± 1,055 vs. 3,145 ± 539%max·s, P < 0.05). In nonsmokers, CVCΔpeakand CVCAUCat the l-NAME site were lower than the Ringer site (CVCΔpeak, 29.5 ± 6.2%max, P < 0.05; and CVCAUC, 5,377 ± 1,109%max·s, P < 0.05), but in smokers, there were no differences between the Ringer and l-NAME sites (CVCΔpeak, 16.8 ± 4.3%max, P = 0.11; and CVCAUC, 2,679 ± 785%max·s, P = 0.30). CVCΔpeakand CVCAUCwere reduced with ketorolac in nonsmokers (CVCΔpeak, 13.3 ± 3.6%max, P < 0.05; and CVCAUC, 1,967 ± 527%max·s, P < 0.05) and smokers (CVCΔpeak, 7.8 ± 1.8%max, P < 0.05; and CVCAUC, 1,246 ± 305%max·s, P < 0.05) and at the combination site in nonsmokers (CVCΔpeak, 15.9 ± 3.1%max, P < 0.05; and CVCAUC, 2,660 ± 512%max·s, P < 0.05) and smokers (CVCΔpeak, 11.5 ± 2.6%max, P < 0.05; and CVCAUC, 1,693 ± 409%max·s, P < 0.05), but the magnitudes were greater in nonsmokers ( P < 0.05). These results suggest that impaired ACh-induced skin vasodilation in young smokers is related to diminished NO- and COX-dependent vasodilation. |
| تدمد: | 1522-1539 0363-6135 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eeb395ee41e66c9fc7f404a8a8a428b1Test https://doi.org/10.1152/ajpheart.00731.2012Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....eeb395ee41e66c9fc7f404a8a8a428b1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221539 03636135 |
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