دورية أكاديمية
Isolation of a Human Betaretrovirus from Patients with Primary Biliary Cholangitis
| العنوان: | Isolation of a Human Betaretrovirus from Patients with Primary Biliary Cholangitis |
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| المؤلفون: | Goubran, Mariam (University of Alberta), Mason, Andrew L. (University of Alberta), Indik, Stanislav (University of Veterinary Medicine Vienna), Wang, Weiwei (University of Alberta), Wong, Gane K-S (University of Alberta), Macintyre, Georgina (University of Alberta), Nuin, Paulo (University of Alberta), Zhang, Guangzhi (University of Alberta), Carpenter, Eric J. (University of Alberta), Bintner, Jasper (University of Alberta), Wasilenko, Shawn T. (University of Alberta), Faschinger, Alexander (University of Veterinary Medicine Vienna) |
| المصدر: | Viruses 14(5) (2022) |
| بيانات النشر: | MDPI |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Mammary-Tumor Virus, Human Breast-Cancer, Env Gene-Sequences, Epithelial-Cells, Mouse, Integration, Cirrhosis, Dna, Identification, Autoimmune |
| الوصف: | A human betaretrovirus (HBRV) has been linked with the autoimmune liver disease, primary biliary cholangitis (PBC), and various cancers, including breast cancer and lymphoma. HBRV is closely related to the mouse mammary tumor virus, and represents the only exogenous betaretrovirus characterized in humans to date. Evidence of infection in patients with PBC has been demonstrated through the identification of proviral integration sites in lymphoid tissue, the major reservoir of infection, as well as biliary epithelium, which is the site of the disease process. Accordingly, we tested the hypothesis that patients with PBC harbor a transmissible betaretrovirus by co-cultivation of PBC patients' lymph node homogenates with the HS578T breast cancer line. Because of the low level of HBRV replication, betaretrovirus producing cells were subcloned to optimize viral isolation and production. Evidence of infection was provided by electron microscopy, RT-PCR, in situ hybridization, cloning of the HBRV proviral genome and demonstration of more than 3400 integration sites. Further evidence of viral transmissibility was demonstrated by infection of biliary epithelial cells. While HBRV did not show a preference for integration proximal to specific genomic features, analyses of common insertion sites revealed evidence of integration proximal to cancer associated genes. These studies demonstrate the isolation of HBRV with features similar to mouse mammary tumor virus and confirm that patients with PBC display evidence of a transmissible viral infection. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | isPartOf:https://phaidra.vetmeduni.ac.at/o:605Test[Publications / University of Veterinary Medicine Vienna]; https://phaidra.vetmeduni.ac.at/o:1909Test |
| DOI: | 10.3390/v14050886 |
| الإتاحة: | https://doi.org/10.3390/v14050886Test https://phaidra.vetmeduni.ac.at/o:1909Test |
| حقوق: | CC BY 4.0 International ; http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.636C2CA1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/v14050886 |
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