دورية أكاديمية
Cofunctioning of bacterial exometabolites drives root microbiota establishment
| العنوان: | Cofunctioning of bacterial exometabolites drives root microbiota establishment |
|---|---|
| المؤلفون: | Getzke, Felix, Amine Hassani, M., Crüsemann, Max, Malisic, Milena, Zhang, Pengfan, Ishigaki, Yuji, Böhringer, Nils, Fernández, Alicia Jiménez, Wang, Lei, Ordon, Jana, Ma, Kawai, Thiergart, Thorsten, Harbort, Christopher J., Wesseler, Hidde, Miyauchi, Shingo, Garrido-Oter, Ruben, Shirasu, Ken, Schäberle, Till Friedrich, Hacquard, Stéphane, Schulze-Lefert, Paul |
| سنة النشر: | 2023 |
| المجموعة: | Publikationsdatenbank der Fraunhofer-Gesellschaft |
| مصطلحات موضوعية: | competition, microbe–microbe interactions, root microbiome, secondary metabolites, synthetic ecology |
| الوصف: | Soil-dwelling microbes are the principal inoculum for the root microbiota, but our understanding of microbe–microbe interactions in microbiota establishment remains fragmentary. We tested 39,204 binary interbacterial interactions for inhibitory activities in vitro, allowing us to identify taxonomic signatures in bacterial inhibition profiles. Using genetic and metabolomic approaches, we identified the antimicrobial 2,4-diacetylphloroglucino l (DAPG) and the iron chelator pyoverdine as exometabolites whose combined functions explain most of the inhibitory activity of the strongly antagonistic Pseudomonas brassicacearum R401. Microbiota reconstitution with a core of Arabidopsis thaliana root commensals in the presence of wild-type or mutant strains revealed a root niche-specific cofunction of these exometabolites as root competence determinants and drivers of predictable changes in the root-associated community. In natural environments, both the corresponding biosynthetic operons are enriched in roots, a pattern likely linked to their role as iron sinks, indicating that these cofunctioning exometabolites are adaptive traits contributing to pseudomonad pervasiveness throughout the root microbiota. ; 120 ; 15 |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 00278424 |
| العلاقة: | Proceedings of the National Academy of Sciences of the United States of America : PNAS; https://publica.fraunhofer.de/handle/publica/447834Test |
| DOI: | 10.1073/pnas.2221508120 |
| الإتاحة: | https://doi.org/10.1073/pnas.2221508120Test https://publica.fraunhofer.de/handle/publica/447834Test |
| حقوق: | open access |
| رقم الانضمام: | edsbas.B7294A79 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00278424 |
|---|---|
| DOI: | 10.1073/pnas.2221508120 |