Genetic ablation of myelin protein zero-like 3 in mice increases energy expenditure, improves glycemic control, and reduces hepatic lipid synthesis
| العنوان: | Genetic ablation of myelin protein zero-like 3 in mice increases energy expenditure, improves glycemic control, and reduces hepatic lipid synthesis |
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| المؤلفون: | Mark R. Wade, John F. Lockwood, Yanyun Chen, Jessica Andrews, Eric D. Hawkins, Michael A. Statnick, Traci A. Czyzyk, Allison E. Sahr, Gabor Varga, Kristine Kay Kikly, Tamer Coskun, Joseph T. Brozinick |
| المصدر: | American Journal of Physiology-Endocrinology and Metabolism. 305:E282-E292 |
| بيانات النشر: | American Physiological Society, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Blood Glucose, Male, medicine.medical_specialty, Physiology, Endocrinology, Diabetes and Metabolism, Blotting, Western, Energy balance, Biology, Real-Time Polymerase Chain Reaction, Weight Gain, Body Temperature, Mice, Physiology (medical), Internal medicine, medicine, Animals, Obesity, Genetic ablation, Triglycerides, Adiposity, Dyslipidemias, Glycemic, Mice, Knockout, Membrane Proteins, Skeletal muscle, Glucose Tolerance Test, Lipids, Diet, Endocrinology, medicine.anatomical_structure, Liver, Energy expenditure, Hepatic lipid, Hyperglycemia, MYELIN PROTEIN ZERO-LIKE 3, Energy Metabolism, Blood Chemical Analysis, Muscle Contraction |
| الوصف: | Obesity continues to be a global health problem, and thus it is imperative that new pathways regulating energy balance be identified. Recently, it was reported: (Hayashi K, Cao T, Passmore H, Jourdan-Le Saux C, Fogelgren B, Khan S, Hornstra I, Kim Y, Hayashi M, Csiszar K. J Invest Dermatol 123: 864–871, 2004) that mice carrying a missense mutation in myelin protein zero-like 3 ( Mpzl3rc) have reduced body weight. To determine how Mpzl3 controls energy balance in vivo, we generated mice deficient in myelin protein zero-like 3 ( Mpzl3-KO). Interestingly, KO mice were hyperphagic yet had reduced body weight and fat mass. Moreover, KO mice were highly resistant to body weight and fat mass gain after exposure to a high-fat, energy-dense diet. These effects on body weight and adiposity were driven, in part, by a pronounced increase in whole body energy expenditure levels in KO mice. KO mice also had reduced blood glucose levels during an intraperitoneal glucose challenge and significant reductions in circulating insulin levels suggesting an increase in insulin sensitivity. In addition, there was an overall increase in oxidative capacity and contractile force in skeletal muscle isolated from KO mice. Hepatic triglyceride levels were reduced by 92% in livers of KO mice, in part due to a reduction in de novo lipid synthesis. Interestingly, Mpzl3 mRNA expression in liver was increased in diet-induced obese mice. Moreover, KO mice exhibited an increase in insulin-stimulated Akt signaling in the liver, further demonstrating that Mpzl3 can regulate insulin sensitivity in this tissue. We have determined that Mpzl3 has a novel physiological role in controlling body weight regulation, energy expenditure, glycemic control, and hepatic triglyceride synthesis in mice. |
| تدمد: | 1522-1555 0193-1849 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b9aa627f3d2df3910bf43c8c5eea107Test https://doi.org/10.1152/ajpendo.00228.2013Test |
| رقم الانضمام: | edsair.doi.dedup.....9b9aa627f3d2df3910bf43c8c5eea107 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221555 01931849 |
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