Gene Expression Pattern in Response to Cholecalciferol Supplementation Highlights Cubilin as a Major Protein of 25(OH)D Uptake in Adipocytes and Male Mice White Adipose Tissue
| العنوان: | Gene Expression Pattern in Response to Cholecalciferol Supplementation Highlights Cubilin as a Major Protein of 25(OH)D Uptake in Adipocytes and Male Mice White Adipose Tissue |
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| المؤلفون: | Catherine Defoort, Esma Karkeni, Lauriane Bonnet, Jean-François Landrier, Jean-Charles Martin, Charlène Couturier, Julie Dalifard, Franck Tourniaire, Ljubica Svilar, Julien Astier |
| المساهمون: | Nutrition, obésité et risque thrombotique (NORT), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix-Marseille Université - Faculté de médecine (AMU MED), Aix Marseille Université (AMU), Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre recherche en CardioVasculaire et Nutrition (C2VN) |
| المصدر: | Endocrinology Endocrinology, 2018, 159 (2), pp.957-966. ⟨10.1210/en.2017-00650⟩ Endocrinology, Endocrine Society, 2018, 159 (2), pp.957-966. ⟨10.1210/en.2017-00650⟩ |
| بيانات النشر: | HAL CCSD, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Adipose tissue, White adipose tissue, Calcitriol receptor, chemistry.chemical_compound, Mice, Endocrinology, Adipocyte, Gene expression, Adipocytes, polycyclic compounds, Vitamin D, Cells, Cultured, Cholecalciferol, MESH: Adipocytes/drug effects, MESH: Adipocytes/metabolism, Female, lipids (amino acids, peptides, and proteins), MESH: Adipose Tissue, White/drug effects, medicine.medical_specialty, Adipose Tissue, White, Receptors, Cell Surface, Biology, 03 medical and health sciences, Internal medicine, 3T3-L1 Cells, medicine, Vitamin D and neurology, Animals, Humans, Nutrition, VDR, Gene Expression Profiling, MESH: Adipose Tissue, White/metabolism, adipocyte, tissu adipeux, souris, protéine majeure, expression génique, expression des gènes, analyse transcriptionnelle, Cubilin, Lipid Metabolism, MESH: 3T3-L1 Cells, Mice, Inbred C57BL, 030104 developmental biology, Metabolism, chemistry, Gene Expression Regulation, Dietary Supplements, Receptors, Calcitriol, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition |
| الوصف: | International audience; It is well established that the active form of vitamin D (i.e., 1,25-dihydroxyvitamin D [1,25(OH)2D]) regulates the expression of genes involved in its own metabolism and transport in the kidney and possibly in the liver. However, little is known about the transcriptional impact of cholecalciferol supplementation on white adipose tissue (WAT) and adipocytes, which are a major site of vitamin D and 25-hydroxyvitamin D [25(OH)D] storage in the organism. To fill this gap, we investigated the impact of cholecalciferol supplementation in WAT via a panel of genes coding for enzymes and proteins involved in vitamin D metabolism and uptake. Mice supplemented with cholecalciferol (15,000 IU/kg of body weight per day) for 4 days showed decreased messenger RNA (mRNA) levels of proteins involved in cholecalciferol metabolism (Cyp24a1, Cyp27a1) and decreased cubilin mRNA levels in WAT. These data were partly confirmed in 3T3-L1 adipocytes incubated with 1,25(OH)2D. The downregulation of cubilin mRNA observed in WAT and in 3T3-L1 was confirmed at the protein level in WAT and at the mRNA level in human primary adipocytes. Vitamin D receptor (VDR) agonist (EB1089) and RNA interference approaches demonstrated that VDR was involved in this regulation. Furthermore, chemical inhibitor and RNA inference analysis demonstrated that cubilin was involved in 25(OH)D uptake by adipocytes. This study established an overall snapshot of the genes regulated by cholecalciferol in mouse WAT and cell-autonomously in adipocytes. We highlighted that the regulation of cubilin expression was mediated by a VDR-dependent mechanism, and we demonstrated that cubilin was involved in 25(OH)D uptake by adipocytes. |
| اللغة: | English |
| تدمد: | 0013-7227 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23b72d54a4233ab33c63bda0d37451ebTest https://amu.hal.science/hal-01998528Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....23b72d54a4233ab33c63bda0d37451eb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00137227 |
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