التفاصيل البيبلوغرافية
| العنوان: |
Engineering Gac/Rsm Signaling Cascade for Optogenetic Induction of the Pathogenicity Switch in Pseudomonas aeruginosa |
| المؤلفون: |
Xinyi Cheng (7624181), Lu Pu (1808686), Shengwei Fu (10911746), Aiguo Xia (4544245), Shuqiang Huang (3859495), Lei Ni (481101), Xiaochen Xing (2021938), Shuai Yang (645019), Fan Jin (466503) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Medicine, Microbiology, Biotechnology, Ecology, Immunology, Infectious Diseases, Computational Biology, Space Science, Biological Sciences not elsewhere classified, GacS, pathogen Pseudomonas aeruginosa, Gac, Caenorhabditis elegans host systems, optogenetic, Pseudomonas aeruginosa strain PAO 1, Pseudomonas aeruginosa, pathogenicity, signal transduction protein, YGS |
| الوصف: |
Bacterial pathogens operate by tightly controlling the pathogenicity to facilitate invasion and survival in host. While small molecule inducers can be designed to modulate pathogenicity to perform studies of pathogen–host interaction, these approaches, due to the diffusion property of chemicals, may have unintended, or pleiotropic effects that can impose limitations on their use. By contrast, light provides superior spatial and temporal resolution. Here, using optogenetics we reengineered GacS of the opportunistic pathogen Pseudomonas aeruginosa , signal transduction protein of the global regulatory Gac/Rsm cascade which is of central importance for the regulation of infection factors. The resultant protein (termed YGS24) displayed significant light-dependent activity of GacS kinases in Pseudomonas aeruginosa . When introduced in the Caenorhabditis elegans host systems, YGS24 stimulated the pathogenicity of the Pseudomonas aeruginosa strain PAO1 in a brain–heart infusion and of another strain, PA14, in slow killing media progressively upon blue-light exposure. This optogenetic system provides an accessible way to spatiotemporally control bacterial pathogenicity in defined hosts, even specific tissues, to develop new pathogenesis systems, which may in turn expedite development of innovative therapeutics. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/journal_contribution/Engineering_Gac_Rsm_Signaling_Cascade_for_Optogenetic_Induction_of_the_Pathogenicity_Switch_in_i_Pseudomonas_aeruginosa_i_/14718362Test |
| DOI: |
10.1021/acssynbio.1c00075.s001 |
| الإتاحة: |
https://doi.org/10.1021/acssynbio.1c00075.s001Test |
| حقوق: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.79CEF41 |
| قاعدة البيانات: |
BASE |
