Chemotherapy-associated steatohepatitis induced by irinotecan: a novel animal model
| العنوان: | Chemotherapy-associated steatohepatitis induced by irinotecan: a novel animal model |
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| المؤلفون: | Marcellus Henrique Loiola Pontes de Souza, Raphael Dias Marques-Neto, Paulo Roberto de Almeida, Karoline S. Aragão, Ronaldo A. Ribeiro, Lorena Guimaraes Nunes, Roberto C. P. Lima-Júnior, Leandro Linhares Leite, Marcelo Leite Vieira Costa, Raul Pinheiro Medeiros, Gerly Anne de Castro Brito, Lucas de Sá Grassi, José Wilson Benevides de Mesquita Neto |
| المصدر: | Cancer Chemotherapy and Pharmacology. 74:711-720 |
| بيانات النشر: | Springer Science and Business Media LLC, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Male, Cancer Research, medicine.medical_specialty, Time Factors, Interleukin-1beta, Irinotecan, Toxicology, Gastroenterology, Mice, Fibrosis, Internal medicine, Weight Loss, medicine, Animals, Humans, Pharmacology (medical), Aspartate Aminotransferases, Survival rate, Peroxidase, Pharmacology, Dose-Response Relationship, Drug, biology, Tumor Necrosis Factor-alpha, business.industry, Alanine Transaminase, medicine.disease, Antineoplastic Agents, Phytogenic, Fatty Liver, Disease Models, Animal, Dose–response relationship, Liver, Oncology, Myeloperoxidase, Toxicity, biology.protein, Camptothecin, Drug Screening Assays, Antitumor, Steatosis, Steatohepatitis, business, medicine.drug |
| الوصف: | Nonalcoholic steatohepatitis (NASH) has been associated with irinotecan (IRI)-based cancer chemotherapy regimens. The purpose of this study was to propose and test a consistent model of IRI-induced NASH, filling a gap in the medical literature. Swiss male mice were distributed in groups (n = 8) and injected with saline (5 mL/kg, i.p.; control) or IRI (25, 50, 75 or 100 mg/kg, i.p.) thrice a week for 7 weeks. Blood samples were collected to measure the serum concentrations of proteins, alanine and aspartate aminotransferases (ALT and AST). Each week animals were euthanized, and the livers were submitted to myeloperoxidase (MPO) assay, lipid dosage, immunohistochemistry for inducible nitric oxide synthase (iNOS), TNF-α and interleukin-1β (IL-1β), and histopathological analysis. Survival rates were also determined. Mice treated with IRI had a significantly (p |
| تدمد: | 1432-0843 0344-5704 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2226d9ace30cf17fe731fa67d46dbb15Test https://doi.org/10.1007/s00280-014-2434-8Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....2226d9ace30cf17fe731fa67d46dbb15 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14320843 03445704 |
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