دورية أكاديمية
3D Spheroids Derived from Human Lipedema ASCs Demonstrated Similar Adipogenic Differentiation Potential and ECM Remodeling to Non-Lipedema ASCs In Vitro
| العنوان: | 3D Spheroids Derived from Human Lipedema ASCs Demonstrated Similar Adipogenic Differentiation Potential and ECM Remodeling to Non-Lipedema ASCs In Vitro |
|---|---|
| المؤلفون: | Al-Ghadban, Sara, Pursell, India A., Diaz, Zaidmara T., Herbst, Karen L., Bunnell, Bruce A. |
| المصدر: | International Journal of Molecular Sciences |
| بيانات النشر: | MDPI |
| سنة النشر: | 2022 |
| المجموعة: | UNTHSC Scholarly Repository (University. of North Texas Health Science Center) |
| مصطلحات موضوعية: | ECM markers, adipogenic differentiation, adipose tissue-derived stem cells (ASCs), inflammation, lipedema, spheroids, Adipocytes / metabolism, Adipogenesis, Adipose Tissue / cytology, Adipose Tissue / metabolism, Cell Culture Techniques / methods, Cell Differentiation, Cell Proliferation, Cells, Cultured, Extracellular Matrix / metabolism, Extracellular Matrix / physiology, Humans, Lipedema / metabolism, Mesenchymal Stem Cells / metabolism, Organoids / metabolism, Stem Cells / metabolism, Tissue Engineering / methods |
| الوصف: | The growth and differentiation of adipose tissue-derived stem cells (ASCs) is stimulated and regulated by the adipose tissue (AT) microenvironment. In lipedema, both inflammation and hypoxia influence the expansion and differentiation of ASCs, resulting in hypertrophic adipocytes and deposition of collagen, a primary component of the extracellular matrix (ECM). The goal of this study was to characterize the adipogenic differentiation potential and assess the levels of expression of ECM-remodeling markers in 3D spheroids derived from ASCs isolated from both lipedema and healthy individuals. The data showed an increase in the expression of the adipogenic genes (ADIPOQ, LPL, PPAR-γ and Glut4), a decrease in matrix metalloproteinases (MMP2, 9 and 11), with no significant changes in the expression of ECM markers (collagen and fibronectin), or integrin A5 in 3D differentiated lipedema spheroids as compared to healthy spheroids. In addition, no statistically significant changes in the levels of expression of inflammatory genes were detected in any of the samples. However, immunofluorescence staining showed a decrease in fibronectin and increase in laminin and Collagen VI expression in the 3D differentiated spheroids in both groups. The use of 3D ASC spheroids provide a functional model to study the cellular and molecular characteristics of lipedema AT. ; This study was supported by the Lipedema foundation. The grant number: 000009. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| تدمد: | 1422-0067 |
| العلاقة: | https://doi.org/10.3390/ijms21218350Test; Al-Ghadban, S., Pursell, I. A., Diaz, Z. T., Herbst, K. L., & Bunnell, B. A. (2020). 3D Spheroids Derived from Human Lipedema ASCs Demonstrated Similar Adipogenic Differentiation Potential and ECM Remodeling to Non-Lipedema ASCs In Vitro. International journal of molecular sciences, 21(21), 8350. https://doi.org/10.3390/ijms21218350Test; https://hdl.handle.net/20.500.12503/31640Test; 21 |
| الإتاحة: | https://doi.org/20.500.12503/31640Test https://doi.org/10.3390/ijms21218350Test https://hdl.handle.net/20.500.12503/31640Test |
| حقوق: | Attribution 4.0 International (CC BY 4.0) ; http://creativecommons.org/licenses/by/4.0Test/ ; © 2020 by the authors. |
| رقم الانضمام: | edsbas.5BB01C80 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14220067 |
|---|