Dermal microdialysis in the dog: in vivo assessment of the effect of cyclosporin A on cutaneous histamine and prostaglandin D2 release
| العنوان: | Dermal microdialysis in the dog: in vivo assessment of the effect of cyclosporin A on cutaneous histamine and prostaglandin D2 release |
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| المؤلفون: | Félix García, Liliana Barandica, Pilar Brazis, Martin K. Church, Geraldine F. Clough, Anna Puigdemont |
| المصدر: | Veterinary Dermatology. 17:169-174 |
| بيانات النشر: | Wiley, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Time Factors, Microdialysis, medicine.medical_treatment, Administration, Oral, Prostaglandin, Pharmacology, Histamine Release, Beagle, Dermatitis, Atopic, chemistry.chemical_compound, Dogs, In vivo, Cyclosporin a, Animals, Medicine, Dog Diseases, Ascaris suum, General Veterinary, biology, Prostaglandin D2, business.industry, biology.organism_classification, Immunosuppressive drug, chemistry, Immunology, Cyclosporine, Female, Dermatologic Agents, business, Immunosuppressive Agents, Histamine |
| الوصف: | Dermal microdialysis, a relatively noninvasive technique, allows investigation of the changes in cellular mediators released during cutaneous allergic responses. This technique was used to evaluate the effect of cyclosporin A, an immunosuppressive drug used for treatment of canine atopic dermatitis, on the cutaneous release of two pro-inflammatory mediators following intradermal allergen challenge. Four beagle dogs spontaneously sensitized to Ascaris suum were treated for 1 month with oral cyclosporin A. At days 0, 15 and 30 of the treatment, dialysis probes were inserted into the skin of the back, and 20 microL of A. suum antigen was injected intradermally at each site. At timed intervals, dialysate was collected and assayed for histamine and prostaglandin D(2) and the wheal area was measured. Mean histamine concentration and wheal area were significantly lower at days 15 and 30 of treatment, compared with day 0. However, prostaglandin D(2) concentration was not significantly reduced. The inhibition in histamine release after intradermal challenge, by cyclosporin, confirms its anti-inflammatory action in the dog. Dermal microdialysis provides a useful tool for investigating canine allergic reactions and their modulation by drugs. |
| تدمد: | 1365-3164 0959-4493 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0d370b487f69e0d85f0b1629eab462e8Test https://doi.org/10.1111/j.1365-3164.2006.00511.xTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....0d370b487f69e0d85f0b1629eab462e8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13653164 09594493 |
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