دورية أكاديمية
A booster dose enhances immunogenicity of the COVID-19 vaccine candidate ChAdOx1 nCoV-19 in aged mice.
| العنوان: | A booster dose enhances immunogenicity of the COVID-19 vaccine candidate ChAdOx1 nCoV-19 in aged mice. |
|---|---|
| المؤلفون: | Silva-Cayetano, Alyssa, Foster, William S, Innocentin, Silvia, Belij-Rammerstorfer, Sandra, Spencer, Alexandra J, Burton, Oliver T, Fra-Bidó, Sigrid, Le Lee, Jia, Thakur, Nazia, Conceicao, Carina, Wright, Daniel, Barrett, Jordan, Evans-Bailey, Nicola, Noble, Carly, Bailey, Dalan, Liston, Adrian, Gilbert, Sarah C, Lambe, Teresa, Linterman, Michelle A |
| بيانات النشر: | Elsevier BV //dx.doi.org/10.1016/j.medj.2020.12.006 Med |
| سنة النشر: | 2021 |
| المجموعة: | Apollo - University of Cambridge Repository |
| مصطلحات موضوعية: | CD8+ T cells, COVID-19, T follicular helper cells, Th1 cells, aging, antibodies, germinal center, immunogenicity, vaccination, Aged, 80 and over, Animals, CD8-Positive T-Lymphocytes, COVID-19 Vaccines, ChAdOx1 nCoV-19, Humans, Mice, SARS-CoV-2 |
| الوصف: | BACKGROUND: The spread of SARS-CoV-2 has caused a worldwide pandemic that has affected almost every aspect of human life. The development of an effective COVID-19 vaccine could limit the morbidity and mortality caused by infection and may enable the relaxation of social-distancing measures. Age is one of the most significant risk factors for poor health outcomes after SARS-CoV-2 infection; therefore, it is desirable that any new vaccine candidates elicit a robust immune response in older adults. METHODS: Here, we use in-depth immunophenotyping to characterize the innate and adaptive immune response induced upon intramuscular administration of the adenoviral vectored ChAdOx1 nCoV-19 (AZD-1222) COVID-19 vaccine candidate in mice. FINDINGS: A single vaccination generates spike-specific Th1 cells, Th1-like Foxp3+ regulatory T cells, polyfunctional spike-specific CD8+ T cells. and granzyme-B-producing CD8 effectors. Spike-specific IgG and IgM are generated from both the early extrafollicular antibody response and the T follicular helper cell-supported germinal center reaction, which is associated with the production of virus-neutralizing antibodies. A single dose of this vaccine generated a similar type of immune response in aged mice but of a reduced magnitude than in younger mice. We report that a second dose enhances the immune response to this vaccine in aged mice. CONCLUSIONS: This study shows that ChAdOx1 nCoV-19 induces both cellular and humoral immunity in adult and aged mice and suggests a prime-boost strategy is a rational approach to enhance immunogenicity in older persons. FUNDING: This study was supported by BBSRC, Lister institute of Preventative Medicine, EPSRC VaxHub, and Innovate UK. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | Print-Electronic; application/pdf |
| اللغة: | English |
| العلاقة: | https://www.repository.cam.ac.uk/handle/1810/317920Test |
| DOI: | 10.17863/CAM.65036 |
| الإتاحة: | https://doi.org/10.17863/CAM.65036Test https://www.repository.cam.ac.uk/handle/1810/317920Test |
| حقوق: | Attribution-NonCommercial-NoDerivatives 4.0 International ; https://creativecommons.org/licenses/by-nc-nd/4.0Test/ |
| رقم الانضمام: | edsbas.4669F16D |
| قاعدة البيانات: | BASE |
| DOI: | 10.17863/CAM.65036 |
|---|