The Golgi apparatus is the main microtubule-organizing center in differentiating skeletal muscle cells
| العنوان: | The Golgi apparatus is the main microtubule-organizing center in differentiating skeletal muscle cells |
|---|---|
| المؤلفون: | Koyo Ide, Kensuke Hayashi, Mika Muko |
| المصدر: | Histochemistry and Cell Biology. 156:273-281 |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Histology, Golgi Apparatus, Cell Line, Mice, 03 medical and health sciences, symbols.namesake, chemistry.chemical_compound, Microtubule, Animals, Muscle, Skeletal, Molecular Biology, Microtubule nucleation, Cell Nucleus, 030102 biochemistry & molecular biology, Myogenesis, Cell Differentiation, Microtubule organizing center, Cell Biology, Golgi apparatus, Brefeldin A, Cell biology, Medical Laboratory Technology, 030104 developmental biology, chemistry, Centrosome, Cytoplasm, symbols, Microtubule-Organizing Center |
| الوصف: | Studies in differentiating skeletal muscle cells in vitro have revealed that the microtubule-organizing center shifts from the centrosome to the perinuclear sites. As the Golgi apparatus surrounds the nucleus in a myotube, it is unclear whether microtubules are nucleated at the nuclear envelope or at the surrounding Golgi apparatus. In this study, we investigated the positional relationship between the microtubule nucleating sites and the Golgi apparatus in C2C12 myotubes and in primary cultured mouse skeletal myotubes. We focused on gaps in the perinuclear Golgi apparatus where the nuclear envelope was not covered with the Golgi apparatus. In microtubule regrowth assay, microtubule regrowth after cold-nocodazole depolymerization of preexisting microtubules was not found at the gap of the perinuclear Golgi apparatus. Most of the microtubule regrowth was detected at the CDK5RAP2 (CDK5 regulatory subunit-associated protein 2)-rich spots on the perinuclear Golgi apparatus. Disruption of the perinuclear Golgi apparatus with brefeldin A treatment eliminated the perinuclear microtubule regrowth. The Golgi apparatus of undifferentiated myoblasts and those at the cytoplasm of myotubes were also the microtubule nucleating sites. From these observations, we concluded that most of the perinuclear microtubule nucleation occurs on the Golgi apparatus surrounding the nucleus. |
| تدمد: | 1432-119X 0948-6143 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb284bf247f14b46c9f13ac859640d82Test https://doi.org/10.1007/s00418-021-01999-6Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....fb284bf247f14b46c9f13ac859640d82 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1432119X 09486143 |
|---|