التفاصيل البيبلوغرافية
| العنوان: |
Additional file 1 of Generation of human chronic wasting disease in transgenic mice |
| المؤلفون: |
Zerui Wang (9359726), Kefeng Qin (495240), Manuel V. Camacho (10454060), Ignazio Cali (367773), Jue Yuan (391711), Pingping Shen (38353), Justin Greenlee (4709119), Qingzhong Kong (147319), James A. Mastrianni (11482299), Wen-Quan Zou (367775) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Medicine, Genetics, Molecular Biology, Neuroscience, Pharmacology, Biotechnology, Immunology, Marine Biology, Cancer, Mental Health, Hematology, Infectious Diseases, Virology, Chemical Sciences not elsewhere classified, Chronic wasting disease (CWD), Prion disease, Prions (PrPSc), Cellular prion protein (PrPC), Serial protein misfolding cyclic amplification (sPMCA) |
| الوصف: |
Additional File 1: Fig. S1. Western blot analysis of PMCA-generated CWD-derived human PrPSc (Cd-HuPrPSc) probed with 6D11 and 3F4. Representative Western blotting of the PK-treated (100 µg/mL) products of 4 rounds of sPMCA that was conducted with (+) or without (−) CWD isolate #7 seeds in the normal human brain homogenates with PrP-129VV (VV#1) probing with 6D11 (a) and 3F4 (b), respectively. The first lane is PrPC from the normal human brain homogenate used as the substrate in sPMCA while the last lane is the PrPSc from the brain of CWD infected elk used as the seed. Both were directly loaded into the gel as the controls. Fig. S2. Western blot analysis of de novo generation of PK-resistant PrPSc by serial PMCA in the presence of PrPC substrate from different species. Representative western blotting of PK-treated (100 µg/mL) products of sPMCA that was conducted with normal brain homogenates from different species in the absence of PrPSc seeds. The normal brain homogenates were from non-CJD cadaver brain tissues with PrP polymorphism methionine (M)/M (n=2, MM#1 and MM#2) or valine (V)/V (n=2, VV#1 and VV#2) at codon 129 of human PrP gene (PRNP), hamster, cervidized Tg mice (TgDeer) and humanized Tg mice (TgMM and TgVV). a and b, 1–4 rounds of sPMCA products with brain homogenates of MM#1, MM#2, VV#1, VV#2, hamster, TgMM, TgVV and TgDeer as the substrate, respectively. c, 5–8 rounds of sPMCA with brain homogenates of MM#1, MM#2, VV#1, VV#2, hamster, TgMM, and TgVV as the substrate, respectively. d, 9–12 rounds of sPMCA with brain homogenates of TgVV, MM#1, MM#2, VV#1, and VV#2 as the substrate, respectively. Blots were probed with the 3F4 antibody. Fig. S3. Western blot analysis of PMCA-generated CWD-derived human PrPSc (Cd-HuPrPSc). Representative Western blotting of the PK-treated (100 µg/mL) products of 2 to 7 rounds of sPMCA that was conducted by seeding CWD isolate #5 or #2 in the normal human brain homogenates with PrP-129VV, respectively. The blot was probed with the 3F4 antibody. Fig. S4. Western blot ... |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/journal_contribution/Additional_file_1_of_Generation_of_human_chronic_wasting_disease_in_transgenic_mice/16682868Test |
| DOI: |
10.6084/m9.figshare.16682868.v1 |
| الإتاحة: |
https://doi.org/10.6084/m9.figshare.16682868.v1Test |
| حقوق: |
CC BY + CC0 |
| رقم الانضمام: |
edsbas.D572A927 |
| قاعدة البيانات: |
BASE |
