Human adenoviral DNA association with nucleosomes containing histone variant H3.3 during the early phase of infection is not dependent on viral transcription or replication
| العنوان: | Human adenoviral DNA association with nucleosomes containing histone variant H3.3 during the early phase of infection is not dependent on viral transcription or replication |
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| المؤلفون: | Andrea N. Giberson, Bratati Saha, Kalisa Campbell, Robin J. Parks, Carin Christou, Kathy L. Poulin |
| المصدر: | Biochemistry and Cell Biology. 96:797-807 |
| بيانات النشر: | Canadian Science Publishing, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Transcription, Genetic, viruses, Virus Replication, Biochemistry, Virus, Histones, 03 medical and health sciences, chemistry.chemical_compound, Transcription (biology), Humans, Nucleosome, Molecular Biology, biology, Adenoviruses, Human, Genetic Variation, Cell Biology, Nucleosomes, Chromatin, Cell biology, 030104 developmental biology, Histone, chemistry, DNA, Viral, biology.protein, Early phase, DNA |
| الوصف: | Adenovirus (Ad) DNA undergoes dynamic changes in protein association as the virus progresses through its replicative cycle. Within the virion, the Ad DNA associates primarily with the virus-encoded, protamine-like protein VII. During the early phase of infection (∼6 h), the viral DNA showed declining association with VII, suggesting that VII was removed from at least some regions of the viral DNA. Within 6 h, the viral DNA was wrapped into a repeating nucleosome-like array containing the histone variant H3.3. Transcription elongation was not required to strip VII from the viral DNA or for deposition of H3.3. H3.1 did not associate with the viral DNA at any point during infection. During the late phase of infection (i.e., active DNA replication ∼12–24 h), association with H3 was dramatically reduced and the repeating nucleosome-like pattern was no longer evident. Thus, we have uncovered some of the changes in nucleoprotein structure that occur during lytic Ad infection. |
| تدمد: | 1208-6002 0829-8211 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2bd01719de38a3540dfbff771edcfa75Test https://doi.org/10.1139/bcb-2018-0117Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....2bd01719de38a3540dfbff771edcfa75 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 12086002 08298211 |
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