False positive Herpes Simplex IgM serology in COVID-19 patients correlates with SARS-CoV-2 IgM/IgG seropositivity
| العنوان: | False positive Herpes Simplex IgM serology in COVID-19 patients correlates with SARS-CoV-2 IgM/IgG seropositivity |
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| المؤلفون: | Laura Vandervore, Eugénie Van Mieghem, Vicky Nowé, Sofie Schouwers, Charlotte Steger, Pascale Abrams, Jozef Van Schaeren, Anissa Meskal, Timon Vandamme |
| المصدر: | Diagnostic microbiology and infectious disease Diagnostic Microbiology and Infectious Disease |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Microbiology (medical), Immunoassay, false positive, SARS-CoV-2, viruses, Pneumonia, Viral, interference, herpes simplex IgM, serology, COVID-19, Herpes Simplex, General Medicine, polyvinylpyrrolidone, Antibodies, Viral, Sensitivity and Specificity, Article, Infectious Diseases, Immunoglobulin M, Immunoglobulin G, Humans, Human medicine, Biology |
| الوصف: | Differentiating COVID-19 from other causes of viral pneumonia, like herpes simplex (HSV), can be complicated by shared clinical and laboratory features. Viral pneumonia is mostly diagnosed based on molecular or serological techniques. Serological immunoassay interferences, often attributed to concurrent appearance of heterologous (viral) immunoglobulins, is well-known, but has not been studied in COVID-19 patients. Following false positive HSV immunoglobulin M (IgM) results in our index patient, 25 other COVID-19 patients were tested for HSV-1/2 IgM with the chemiluminescent Liaison assay and Euroimmun enzyme-linked immunosorbent assay. Forty-five percent of COVID-19 patients tested positive for HSV IgM with Liaison. No HSV indices were positive with Euroimmun enzyme-linked immunosorbent assay, suggesting immunoassay interference. Significant correlation between HSV IgM and SARS-CoV-2 IgM/IgG positivity was found. Adding 0.5% polyvinylpyrrolidone, inhibiting non-specific solid-phase adsorption, abolished interference in 22% of false positive cases, suggesting interference caused by solid-phase reactive IgM. Hence, serologic immunoassay results should be interpreted with caution in COVID-19 patients. (c) 2022 Elsevier Inc. All rights reserved. |
| اللغة: | English |
| تدمد: | 0732-8893 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a58341957148774a19663f954bd946edTest https://hdl.handle.net/10067/1857780151162165141Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a58341957148774a19663f954bd946ed |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 07328893 |
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