التفاصيل البيبلوغرافية
| العنوان: |
Analysis of Fixed and Live Single Cells Using Optical Photothermal Infrared with Concomitant Raman Spectroscopy |
| المؤلفون: |
Alice Spadea (6655253), Joanna Denbigh (10155963), M. Jayne Lawrence (1687354), Mustafa Kansiz (10061573), Peter Gardner (236730) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Cell Biology, Genetics, Cancer, Inorganic Chemistry, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, data acquisition, breast cancer cell line MDA-MB -231, Live Single Cells, paper reports, QCL, quantum cascade laser, pancreatic cancer cell line MIA PaCa -2, subcellular structures, Raman spectra, OPO, photothermal method, lipid-rich bodies, submicron scale, Concomitant Raman Spectroscopy, vibrational profile, model cells, excitation sources, Optical Photothermal, excitation location, O-PTIR |
| الوصف: |
This paper reports the first use of a novel completely optically based photothermal method (O-PTIR) for obtaining infrared spectra of both fixed and living cells using a quantum cascade laser (QCL) and optical parametric oscillator (OPO) laser as excitation sources, thus enabling all biologically relevant vibrations to be analyzed at submicron spatial resolution. In addition, infrared data acquisition is combined with concomitant Raman spectra from exactly the same excitation location, meaning the full vibrational profile of the cell can be obtained. The pancreatic cancer cell line MIA PaCa-2 and the breast cancer cell line MDA-MB-231 are used as model cells to demonstrate the capabilities of the new instrumentation. These combined modalities can be used to analyze subcellular structures in both fixed and, more importantly, live cells under aqueous conditions. We show that the protein secondary structure and lipid-rich bodies can be identified on the submicron scale. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/journal_contribution/Analysis_of_Fixed_and_Live_Single_Cells_Using_Optical_Photothermal_Infrared_with_Concomitant_Raman_Spectroscopy/14046774Test |
| DOI: |
10.1021/acs.analchem.0c04846.s001 |
| الإتاحة: |
https://doi.org/10.1021/acs.analchem.0c04846.s001Test |
| حقوق: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.C5D71B12 |
| قاعدة البيانات: |
BASE |
