دورية أكاديمية
Bacillus cereus cereolysin O induces pyroptosis in an undecapeptide-dependent manner
العنوان: | Bacillus cereus cereolysin O induces pyroptosis in an undecapeptide-dependent manner |
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المؤلفون: | Yujian Wang, Jingchang Luo, Xiaolu Guan, Yan Zhao, Li Sun |
المصدر: | Cell Death Discovery, Vol 10, Iss 1, Pp 1-9 (2024) |
بيانات النشر: | Nature Publishing Group, 2024. |
سنة النشر: | 2024 |
المجموعة: | LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens LCC:Cytology |
مصطلحات موضوعية: | Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282, Cytology, QH573-671 |
الوصف: | Abstract Bacillus cereus is a clinically significant foodborne pathogen that causes severe gastrointestinal and non-gastrointestinal disease. Cereolysin O (CLO) is a putative virulence factor of B. cereus, and its function remains to be investigated. In this study, we examined the biological activity of CLO from a deep sea B. cereus isolate. CLO was highly toxic to mammalian cells and triggered pyroptosis through NLRP3 inflammasome-mediated caspase 1 and gasdermin D activation. CLO-induced cell death involved ROS accumulation and K+ efflux, and was blocked by serum lipids. CLO bound specifically to cholesterol, and this binding was essential to CLO cytotoxicity. The structural integrity of the three tryptophan residues in the C-terminal undecapeptide was vital for CLO to interact with membrane lipids and cause membrane perforation. Taken together, these results provided new insights into the molecular mechanism of B. cereus CLO-mediated cytotoxicity. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2058-7716 |
العلاقة: | https://doaj.org/toc/2058-7716Test |
DOI: | 10.1038/s41420-024-01887-7 |
الوصول الحر: | https://doaj.org/article/d9aa71cee06a44f7b259d7bb1b4f7a1aTest |
رقم الانضمام: | edsdoj.9aa71cee06a44f7b259d7bb1b4f7a1a |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 20587716 |
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DOI: | 10.1038/s41420-024-01887-7 |