Effect of brachytherapy on NF-κB and VEGF in gastric carcinoma xenografts
| العنوان: | Effect of brachytherapy on NF-κB and VEGF in gastric carcinoma xenografts |
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| المؤلفون: | Xiao-Gang Li, Wang Mingchun, Bo Li, Mao Wenyuan, Jin Wendi, Kaiyuan Luo, Wan-Fu Zhang |
| المصدر: | Oncology Reports. 32:635-640 |
| بيانات النشر: | Spandidos Publications, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Vascular Endothelial Growth Factor A, Cancer Research, Pathology, medicine.medical_specialty, medicine.medical_treatment, Brachytherapy, Cell, Apoptosis, Protein Serine-Threonine Kinases, Biology, Flow cytometry, Mice, Stomach Neoplasms, Cell Line, Tumor, medicine, Animals, Humans, Oncogene, medicine.diagnostic_test, Cell Cycle, Cancer, General Medicine, Cell cycle, medicine.disease, Xenograft Model Antitumor Assays, Molecular medicine, Gene Expression Regulation, Neoplastic, medicine.anatomical_structure, Oncology, Cancer research |
| الوصف: | Iodine-125 (125I) seed irradiation can be used as an important supplementary treatment for unresectable advanced gastric cancer. However, the radiobiological mechanism underlying brachytherapy remains unclear. Therefore, we investigated the influence of continuous and low-energy 125I irradiation on the cell cycle distribution, apoptosis, expression of NF-κB and VEGF and tumor growth in a human gastric cancer xenograft model. To create an animal model of gastric cancer, SGC-7901 cells were surgically implanted into mice. The 60 mice bearing SGC-7901 gastric cancer xenografts were randomly separated into 2 groups. Sham seeds (0 mCi) were implanted into the control group (n=30); 125I seeds (0.6 mCi) were implanted into the treatment group (n=30). At 28 days after irradiation, apoptosis was detected by flow cytometry. fluorescence micrograph detected intense VEGF and NF-κB immunofluorescence in the tumor samples, and changes in NF-κB and VEGF mRNA and protein expression were assessed by real-time PCR and western blot analysis, respectively. The tumor volume and weight were measured 0-28 days after 125I seed implantation. 125I seed irradiation induced significant apoptosis and G2/M phase arrest. Reduction in the intensities of VEGF and NF-κB immunofluorescence in tumor vessels was observed after treatment. NF-κB and VEGF mRNA and protein expression levels were substantially lower in the implantation treatment group than in the control group. Consequently, 125I seed implantation inhibited cancer growth and reduced cancer volume. The present study revealed that 125I seed irradiation significantly induced apoptosis and cell cycle arrest in the human gastric cancer xenografts. 125I-induced changes in NF-κB and VEGF expression are suggested as potential mechanisms underlying effective brachytherapy. |
| تدمد: | 1791-2431 1021-335X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::744bd00dacc9d6b911e4c206ae5abd7eTest https://doi.org/10.3892/or.2014.3255Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....744bd00dacc9d6b911e4c206ae5abd7e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17912431 1021335X |
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