Nanodrug delivery platform for glucocorticoid use in skeletal muscle injury
| العنوان: | Nanodrug delivery platform for glucocorticoid use in skeletal muscle injury |
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| المؤلفون: | Travis Webb, Kathleen Halasz, Robert Weigel, Rohini Nimbalkar, Kalyan C. Chapalamadugu, Srinivas M. Tipparaju, Jared Tur, Marco Brotto, Yashwant Pathak, Todd Daviau, Shannon Kelly, Vijaykumar Sutariya, Janice Cacace |
| المصدر: | Canadian Journal of Physiology and Pharmacology. 96:681-689 |
| بيانات النشر: | Canadian Science Publishing, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Cell Survival, Physiology, Drug Compounding, Biological Availability, Inflammation, 02 engineering and technology, Pharmacology, Dexamethasone, Cell Line, Myoblasts, Mice, 03 medical and health sciences, chemistry.chemical_compound, Microscopy, Electron, Transmission, Polylactic Acid-Polyglycolic Acid Copolymer, Physiology (medical), Lactate dehydrogenase, medicine, Animals, Myocyte, Lactic Acid, Viability assay, Muscle, Skeletal, Glucocorticoids, Wound Healing, Myositis, Skeletal muscle, General Medicine, 021001 nanoscience & nanotechnology, Rats, Drug Liberation, PLGA, 030104 developmental biology, medicine.anatomical_structure, chemistry, Toxicity, Nanoparticles, medicine.symptom, 0210 nano-technology, Polyglycolic Acid, medicine.drug |
| الوصف: | Glucocorticoids are utilized for their anti-inflammatory properties in the skeletal muscle and arthritis. However, the major drawback of use of glucocorticoids is that it leads to senescence and toxicity. Therefore, based on the idea that decreasing particle size allows for increased surface area and bioavailability of the drug, in the present study, we hypothesized that nanodelivery of dexamethasone will offer increased efficacy and decreased toxicity. The dexamethasone-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles were prepared using nanoprecipitation method. The morphological characteristics of the nanoparticles were studied under scanning electron microscope. The particle size of nanoparticles was 217.5 ± 19.99 nm with polydispersity index of 0.14 ± 0.07. The nanoparticles encapsulation efficiency was 34.57% ± 1.99% with in vitro drug release profile exhibiting a sustained release pattern over 10 days. We identified improved skeletal muscle myoblast performance with improved closure of the wound along with increased cell viability at 10 nmol/L nano-dexamethasone-PLGA. However, dexamethasone solution (1 μmol/L) was injurious to cells because the migration efficiency was decreased. In addition, the use of dexamethasone nanoparticles decreased lipopolysaccharide-induced lactate dehydrogenase release compared with dexamethasone solution. Taken together, the present study clearly demonstrates that delivery of PLGA-dexamethasone nanoparticles to the skeletal muscle cells is beneficial for treating inflammation and skeletal muscle function. |
| تدمد: | 1205-7541 0008-4212 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::69b8af8b24913997b212efec48483875Test https://doi.org/10.1139/cjpp-2017-0795Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....69b8af8b24913997b212efec48483875 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 12057541 00084212 |
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