التفاصيل البيبلوغرافية
| العنوان: |
Lymphatic endothelium stimulates melanoma metastasis and invasion via MMP14-dependent Notch3 and beta 1-integrin activation |
| المؤلفون: |
Pekkonen, Pirita, Alve, Sanni, Balistreri, Giuseppe, Gramolelli, Silvia, Tatti-Bugaeva, Olga, Paatero, Ilkka, Niiranen, Otso, Tuohinto, Krista, Perala, Nina, Taiwo, Adewale, Zinovkina, Nadezhda, Repo, Pauliina, Icay, Katherine, Ivaska, Joanna, Saharinen, Pipsa, Hautaniemi, Sampsa, Lehti, Kaisa, Ojala, Paivi M. |
| المساهمون: |
Research Programs Unit, Translational Cancer Biology (TCB) Research Programme, Genome-Scale Biology (GSB) Research Program, Pipsa Ilona Saharinen / Principal Investigator, Sampsa Hautaniemi / Principal Investigator, Bioinformatics, Kaisa Irene Lehti / Principal Investigator, Päivi Ojala / Research director |
| بيانات النشر: |
ELIFE SCIENCES PUBLICATIONS LTD |
| سنة النشر: |
2018 |
| المجموعة: |
Helsingfors Universitet: HELDA – Helsingin yliopiston digitaalinen arkisto |
| مصطلحات موضوعية: |
TO-MESENCHYMAL TRANSITION, TUMOR PROGRESSION, NODE METASTASIS, CELL-ADHESION, CANCER, METALLOPROTEINASES, EXPRESSION, PLATFORM, VESSELS, CCR7, 3111 Biomedicine |
| الوصف: |
Lymphatic invasion and lymph node metastasis correlate with poor clinical outcome in melanoma. However, the mechanisms of lymphatic dissemination in distant metastasis remain incompletely understood. We show here that exposure of expansively growing human WM852 melanoma cells, but not singly invasive Bowes cells, to lymphatic endothelial cells (LEC) in 3D co-culture facilitates melanoma distant organ metastasis in mice. To dissect the underlying molecular mechanisms, we established LEC co-cultures with different melanoma cells originating from primary tumors or metastases. Notably, the expansively growing metastatic melanoma cells adopted an invasively sprouting phenotype in 3D matrix that was dependent on MMP14, Notch3 and beta 1-integrin. Unexpectedly, MMP14 was necessary for LEC-induced Notch3 induction and coincident beta 1-integrin activation. Moreover, MMP14 and Notch3 were required for LEC-mediated metastasis of zebrafish xenografts. This study uncovers a unique mechanism whereby LEC contact promotes melanoma metastasis by inducing a reversible switch from 3D growth to invasively sprouting cell phenotype. ; Peer reviewed |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
Terveyden Tutkimuksen Toimi-kunta 307366 Johanna Ivaska Pipsa Saharinen Paivi M Ojala; Pekkonen , P , Alve , S , Balistreri , G , Gramolelli , S , Tatti-Bugaeva , O , Paatero , I , Niiranen , O , Tuohinto , K , Perala , N , Taiwo , A , Zinovkina , N , Repo , P , Icay , K , Ivaska , J , Saharinen , P , Hautaniemi , S , Lehti , K & Ojala , P M 2018 , ' Lymphatic endothelium stimulates melanoma metastasis and invasion via MMP14-dependent Notch3 and beta 1-integrin activation ' , eLife , vol. 7 , 32490 . https://doi.org/10.7554/eLife.32490Test; ORCID: /0000-0002-7749-2694/work/44998566; ORCID: /0000-0002-9257-9376/work/90907025; 85049593514; cba1f277-1169-4721-b243-c8325363fca1; http://hdl.handle.net/10138/235268Test; 000431114900001 |
| الإتاحة: |
http://hdl.handle.net/10138/235268Test |
| حقوق: |
cc_by ; openAccess ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edsbas.C5B7D9E |
| قاعدة البيانات: |
BASE |
