High gestational folic acid supplementation prevents hypoxia‐ischemia‐induced caspase‐3 augmenting without changing synapsin and H3 methylation levels in the rat hippocampus
| العنوان: | High gestational folic acid supplementation prevents hypoxia‐ischemia‐induced caspase‐3 augmenting without changing synapsin and H3 methylation levels in the rat hippocampus |
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| المؤلفون: | Bruna Ferrary Deniz, Patrícia Maidana Miguel, Lenir Orlandi Pereira, Isabela R Fernandes, Loise Bronauth, Alysson R. Muotri, Heloísa Deola Confortim |
| المصدر: | International Journal of Developmental Neuroscience. 81:510-519 |
| بيانات النشر: | Wiley, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, medicine.medical_specialty, Apoptosis, Caspase 3, Hippocampus, Histones, 03 medical and health sciences, Histone H3, Folic Acid, 0302 clinical medicine, Developmental Neuroscience, Pregnancy, Internal medicine, Histone methylation, Animals, Medicine, Hippocampus (mythology), Epigenetics, Rats, Wistar, 030304 developmental biology, 0303 health sciences, business.industry, Synapsin, DNA Methylation, Synapsins, medicine.disease, Rats, Perinatal asphyxia, B vitamins, Endocrinology, Hypoxia-Ischemia, Brain, Female, business, 030217 neurology & neurosurgery, Developmental Biology |
| الوصف: | Perinatal asphyxia is a peripartum event that can cause permanent sequelae to the newborns, affecting the brain development. Recently, it has been demonstrated that epigenetics mechanisms play an important role in this injury and that folic acid (FA) supplementation during pregnancy can affect these epigenetics modifications as well as gene expression. We have identified both positive and negative effects of FA treatment in rats submitted to a model of neonatal hypoxia-ischemia (HI). Considering that FA supplementation is already used in pregnant women and that HI occurs in the peripartum period, this study was designated to evaluate how gestational FA supplementation and neonatal HI affect: apoptosis (caspase-3) and expression of synaptic proteins (synapsin and PSD-95) and the methylation of histone H3 lysine (K) 4 and 27 in the rat hippocampus. Pregnant Wistar rats were divided according to the diets: standard (SD), supplemented with 2 mg/kg of FA or with 20 mg/kg of FA. HI procedure was performed at the 7th PND. Protein expression and H3 methylation were evaluated at the 60th PND in the rats' hippocampus. Neonatal HI increased caspase-3 expression decreased synapsin expression and reduced H3K4me2, -me3 and H3K27me2, -me3 in the ipsilateral hippocampus. FA only prevented the augment in caspase-3 expression. In conclusion, neonatal HI caused lasting effects on caspase-3-mediated cell death (prevented by the FA) and synaptic proteins in the rats' hippocampus. This is the first study to show that histone modifications may contribute to these pathological findings in the hippocampus of HI animals. |
| تدمد: | 1873-474X 0736-5748 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54eca6a2106cedfd5f38229df6f4878fTest https://doi.org/10.1002/jdn.10132Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....54eca6a2106cedfd5f38229df6f4878f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1873474X 07365748 |
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