دورية أكاديمية
TiO2 nanostructured implant surface-mediated M2c polarization of inflammatory monocyte requiring intact cytoskeleton rearrangement
| العنوان: | TiO2 nanostructured implant surface-mediated M2c polarization of inflammatory monocyte requiring intact cytoskeleton rearrangement |
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| المؤلفون: | Zhaoyue Fu, Yongli Hou, Håvard Jostein Haugen, Xutao Chen, Kang Tang, Liang Fang, Yong Liu, Shu Zhang, Qianli Ma, Lihua Chen |
| المصدر: | Journal of Nanobiotechnology, Vol 21, Iss 1, Pp 1-16 (2023) |
| بيانات النشر: | BMC, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biotechnology LCC:Medical technology |
| مصطلحات موضوعية: | TiO2, Cytoskeleton, Inflammatory monocyte (iMos), Simulated microgravity (SMG), Macrophage polarization, Biotechnology, TP248.13-248.65, Medical technology, R855-855.5 |
| الوصف: | Abstract Background Microgravity directly disturbs the reorganization of the cytoskeleton, exerting profound effects on the physiological process of macrophages. Although it has been established that macrophage M1/M2 polarization could be manipulated by the surface nanostructure of biomaterial in our previous study under normal gravity, how will inflammatory monocytes (iMos)-derived macrophages respond to diverse nanostructured Ti surfaces under normal gravity or microgravity remains unrevealed. Results In this study, Cytochalasin D, a cytoskeleton relaxant, was employed to establish the simulated microgravity (SMG) environment. Our results showed that human iMos polarized into M2c macrophages on NT5 surface but M1 type on NT20 surface with divergent inflammatory phenotypes according to the profile of macrophage polarization featured molecules under normal gravity. However, such manipulative effects of NTs surfaces on iMos-derived macrophages were strikingly weakened by SMG, characterized by the altered macrophage morphology, changed cytokine secretion profile, and decreased cell polarization capacity. Conclusions To our knowledge, this is the first metallic implantable material study focusing on the functions of specific monocyte subsets and its crucial role of the cytoskeleton in materials-mediated host immune response, which enriches our mechanism knowledge about the crosstalk between immunocytes and biomaterials. The results obtained in the present study may also provide potential targets and strategies for biomaterial development and clinical treatment via precise immune-regulation under normal gravity and microgravity. Graphic Abstract |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1477-3155 |
| العلاقة: | https://doaj.org/toc/1477-3155Test |
| DOI: | 10.1186/s12951-022-01751-9 |
| الوصول الحر: | https://doaj.org/article/99489b9b5ecf41478d99c1813297ce7aTest |
| رقم الانضمام: | edsdoj.99489b9b5ecf41478d99c1813297ce7a |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14773155 |
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| DOI: | 10.1186/s12951-022-01751-9 |