دورية أكاديمية
Characterization of Blood Mucosal Associated Invariant T ( MAIT ) cells in Axial Spondyloarthritis and of resident MAITs from control axial enthesis
| العنوان: | Characterization of Blood Mucosal Associated Invariant T ( MAIT ) cells in Axial Spondyloarthritis and of resident MAITs from control axial enthesis |
|---|---|
| المؤلفون: | Rosine, Nicolas, Rowe, Hannah, Koturan, Surya, Yahia‐cherbal, Hanane, Leloup, Claire, Watad, Abdulla, Berenbaum, Francis, Sellam, Jeremie, Dougados, Maxime, Aimanianda, Vishukumar, Cuthbert, Richard, Bridgewood, Charlie, Newton, Darren, Bianchi, Elisabetta, Rogge, Lars, Mcgonagle, Dennis, Miceli‐richard, Corinne |
| المساهمون: | Immunorégulation - Immunoregulation, Institut Pasteur Paris (IP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Cité (UPCité), University of Leeds, Sorbonne Université (SU), CHU Saint-Antoine AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Cochin AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Mycologie moléculaire - Molecular Mycology, Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), St James's University Hospital, Leeds Teaching Hospitals NHS Trust, Institut Pasteur Paris (IP), DMG is funded by the Leeds NIHR Biomedical Research Centre. Novartis UK Investigator Initiated non-clinical research funding support (HR, CB). AW was funded by the Celgene supported PARTNER fellowship program. Research funded by a Pfizer investigator initiated research grant (RJC) NR was funded by a Poste d’Accueil APHP and a grant fromSociété Française de Rhumatologie. This study was supported by grants from MSD Avenir (Project iCARE-SpA), a Sanofi Innovation award Europe, FOREUM Foundation for Research in Rheumatology, and from the Société Française de Rhumatologie. |
| المصدر: | ISSN: 2326-5205. |
| بيانات النشر: | HAL CCSD Wiley |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | IL-17A, IL-17F, IL-17 producing cells, axial spondyloarthritis, MAITs, [SDV.IMM]Life Sciences [q-bio]/Immunology |
| الوصف: | International audience ; ObjectivesThe importance of IL-17A in the pathogenesis of Axial Spondyloarthritis (AxSpA) has been demonstrated by the success of IL-17A blockade. However, the nature of the cell populations producing this important pro-inflammatory cytokine remains poorly defined. Accordingly, we characterized major IL-17A-producing blood cell populations in AxSpA, with reference to entheseal biology, with a focus on mucosal associated invariant T-cells, (MAITs) a population known to be capable of producing IL-17.MethodsIL-17A production from 5 sorted peripheral blood cell populations, namely MAITs, γδ T-cells, CD4+T-cells, CD8+T-cells and neutrophils was evaluated pre- and post-stimulation with PMA, the calcium ionophore A23187 and β1,3 glucan. IL-17A transcript and protein expression were determined using nCounter Technology and ultra-sensitive SimoA technology, respectively. MAITs from control human axial entheses (n=5) were further characterized by flow cytometric immunophenotyping, qPCR and IL-17 production assessed following stimulation.ResultsIn blood, on a per cell basis, MAIT cells produced the highest amount of IL-17A compared to CD4+ (p <0.01), CD8+ (p <0.0001) and γδ T-cells (p <0.0001). IL-17A was not produced by neutrophils. Gene expression analysis also showed significantly higher IL17A and IL23R expression in MAIT cells. Stimulation of blood MAIT cells with αCD3/CD28 and IL-7 and/or IL-18 induced strong expression of IL17F. MAITs were present in the normal enthesis and showed elevated AHR, JAK1, STAT4 and TGFB1 transcript expression with inducible IL-17A protein. IL-18 protein expression was evident in spinal enthesis digests.ConclusionBoth blood MAITs and resident MAITs in axial entheses contribute to IL-17 production and may play important roles in AxSpA pathogenesis. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/35166073; pasteur-03670096; https://pasteur.hal.science/pasteur-03670096Test; https://pasteur.hal.science/pasteur-03670096/documentTest; https://pasteur.hal.science/pasteur-03670096/file/Arthritis%20Rheumatology%20-%202022%20-%20Rosine%20-%20Characterization%20of%20Blood%20Mucosal%20Associated%20Invariant%20T%20MAIT%20cells%20in%20Axial.pdfTest; PUBMED: 35166073 |
| DOI: | 10.1002/art.42090 |
| الإتاحة: | https://doi.org/10.1002/art.42090Test https://pasteur.hal.science/pasteur-03670096Test https://pasteur.hal.science/pasteur-03670096/documentTest https://pasteur.hal.science/pasteur-03670096/file/Arthritis%20Rheumatology%20-%202022%20-%20Rosine%20-%20Characterization%20of%20Blood%20Mucosal%20Associated%20Invariant%20T%20MAIT%20cells%20in%20Axial.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.884DEE4F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/art.42090 |
|---|