Interassay and interobserver comparability study of four programmed death-ligand 1 (PD-L1) immunohistochemistry assays in triple-negative breast cancer
| العنوان: | Interassay and interobserver comparability study of four programmed death-ligand 1 (PD-L1) immunohistochemistry assays in triple-negative breast cancer |
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| المؤلفون: | Alexander Hapfelmeier, Sebastian Foersch, Siranush Karapetyan, Daniel-Christoph Wagner, Katja Steiger, Kristina Schwamborn, Marion Kiechle, Wilko Weichert, Wilfried Roth, Dirk Oettler, Aurelia Noske |
| المصدر: | Breast, Vol 60, Iss, Pp 238-244 (2021) The Breast : Official Journal of the European Society of Mastology |
| بيانات النشر: | Elsevier, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Oncology, CPS, combined positive score, TC, tumor cells, ICI, immune checkpoint inhibitor, Triple Negative Breast Neoplasms, B7-H1 Antigen, Medicine, HER2, human epidermal growth factor receptor 2, Triple-negative breast cancer, RC254-282, ICC, intraclass correlation coefficient, biology, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, General Medicine, MSI, microsatellite instability, Immunohistochemistry, pCR, pathological complete response, PFS, progression-free survival, Original Article, IC-Score, IC, immune cells, IHC, immunohistochemistry, Programmed death, PD-L1, medicine.medical_specialty, Concordance, TNBC, triple-negative breast cancer, OS, overall survival, Breast cancer, Internal medicine, TPS, tumor proportion score, Biomarkers, Tumor, Humans, Programmed death-ligand 1, Reproducibility, business.industry, Reproducibility of Results, medicine.disease, ITT, intention to treat, CI, confidence interval, PD-L1, programmed death-ligand 1, biology.protein, Surgery, CPS, business, Kappa, TMB, tumor mutational burden |
| الوصف: | Different immunohistochemical programmed death-ligand 1 (PD-L1) assays and scorings have been reported to yield variable results in triple-negative breast cancer (TNBC). We compared the analytical concordance and reproducibility of four clinically relevant PD-L1 assays assessing immune cell (IC) score, tumor proportion score (TPS), and combined positive score (CPS) in TNBC. Primary TNBC resection specimens (n = 104) were stained for PD-L1 using VENTANA SP142, VENTANA SP263, DAKO 22C3, and DAKO 28–8. PD-L1 expression was scored according to guidelines on virtual whole slide images by four trained readers. The mean PD-L1 positivity at IC-score ≥1% and CPS ≥1 ranged between 53% and 75% with the highest positivity for SP263 and comparable levels for 22C3, 28–8, and SP142. Inter-assay agreement was good between 28–8 and 22C3 across all scores and cut-offs (kappa 0.68–0.74) and for both assays with SP142 at IC-score ≥1% and CPS ≥1 (kappa 0.61–0.67). The agreement between SP263 and all other assays was substantially lower for all scores. Inter-reader agreement for each assay was good to excellent for IC-score ≥1% (kappa 0.73–0.78) and CPS ≥1 (kappa 0.68–0.74), fair to good for CPS ≥10 (kappa 0.52–0.67) and TPS ≥1% (kappa 0.53–0.72). The percentage of overlapping cases in the positive/negative category was >90% between IC-score ≥1% and CPS ≥1 but below when comparing IC-score ≥1% with CPS ≥10. We demonstrate an overall good inter-reader agreement for all PD-L1 assays in TNBC along with assay specific differences in positivity and concordances, which may aid to select the right test strategy in routine diagnostics. Highlights • Different PD-L1 IHC assays and scorings may show variable results in TNBC. • Overall good assay concordance between SP142, 22C3, and 28–8 at IC-score 1%. • Overall good assay concordance between SP142, 22C3, and 28–8 at CPS 1. • SP142 is less optimal for CPS assessment at higher cut-offs. • SP263 assay is not interchangeable with the other three PD-L1 assays. |
| اللغة: | English |
| تدمد: | 1532-3080 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::05a50b5eac3f8c4ff158e8c3f6dfcb0cTest http://www.sciencedirect.com/science/article/pii/S0960977621009851Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....05a50b5eac3f8c4ff158e8c3f6dfcb0c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15323080 |
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