NDM-producing Enterobacteriaceae in Poland, 2012–14: inter-regional outbreak ofKlebsiella pneumoniaeST11 and sporadic cases
| العنوان: | NDM-producing Enterobacteriaceae in Poland, 2012–14: inter-regional outbreak ofKlebsiella pneumoniaeST11 and sporadic cases |
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| المؤلفون: | I. Gawryszewska, Anna Baraniak, Radosław Izdebski, D. Żabicka, T. Ozorowski, Marek Gniadkowski, Katarzyna Bojarska, M. Szarata, N. Pewińska, E Literacka, A. Milner, M. Herda, Janusz Fiett, Waleria Hryniewicz, H. Tomczak |
| المصدر: | Journal of Antimicrobial Chemotherapy. 71:85-91 |
| بيانات النشر: | Oxford University Press (OUP), 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Adult, DNA, Bacterial, Male, 0301 basic medicine, Microbiology (medical), Adolescent, Gene Transfer, Horizontal, Genotype, Klebsiella pneumoniae, 030106 microbiology, Polymerase Chain Reaction, beta-Lactamases, Disease Outbreaks, Microbiology, 03 medical and health sciences, Plasmid, Pulsed-field gel electrophoresis, Humans, Outpatient clinic, Pharmacology (medical), Typing, Pharmacology, biology, Nucleic Acid Hybridization, Outbreak, Sequence Analysis, DNA, Middle Aged, biology.organism_classification, Klebsiella Infections, New Delhi metallo-beta-lactamase 1, Molecular Typing, Infectious Diseases, biology.protein, Multilocus sequence typing, Female, Poland, Plasmids |
| الوصف: | Objectives The objective of this study was to characterize New Delhi metallo-β-lactamase (NDM)-producing Enterobacteriaceae isolates reported in Poland in 2012-14. Methods Representative isolates were typed by PFGE and MLST. NDM and other β-lactamase genes were amplified and sequenced. Plasmids with blaNDM genes were analysed by nuclease S1 plus hybridization profiling, by transfer assays and by PCR-based replicon typing. The blaNDM genetic context was studied by PCR mapping assays. Results Of 374 cases of infection/colonization with NDM-positive Enterobacteriaceae identified in 2012-14, 370 cases in 40 hospitals, 10 outpatient clinics and 1 nursing home were associated with a Klebsiella pneumoniae outbreak with epicentres in Poznan and Warsaw. The outbreak strain of K. pneumoniae ST11 was similar to an isolate from the Czech Republic from 2013. Like the Czech strain, many of the isolates had two blaNDM-1-carrying IncFII- and IncR-type plasmids of variable size, sharing a blaNDM-1-containing segment. The early isolates also produced CTX-M-15 co-encoded by the IncR-type plasmids, and differentiated later by extensive plasmid rearrangements. Four other NDM cases were reported in 2013, three being associated with arrivals from Montenegro, India or Afghanistan. The Indian Escherichia coli ST448 NDM-5 isolate revealed similarity to a recent isolate from Spain, including the blaNDM genetic context observed previously in E. coli strains in Poland and France (of Congolese and Indian origins, respectively). The Afghani Proteus mirabilis was the second isolate of this species with a chromosomal blaNDM-1 location. Conclusions The largest NDM outbreak in a non-endemic country has been observed, being an alarming phenomenon in resistance epidemiology in Poland. |
| تدمد: | 1460-2091 0305-7453 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8545ddd99dbd301cf7c2c202be28d8e6Test https://doi.org/10.1093/jac/dkv282Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8545ddd99dbd301cf7c2c202be28d8e6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602091 03057453 |
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