التفاصيل البيبلوغرافية
| العنوان: |
Mineral crystal thickness in calcified cartilage and subchondral bone in healthy and osteoarthritic human knees |
| المؤلفون: |
Finnilä, M. A. (Mikko A.J.), Das Gupta, S. (Shuvashis), Turunen, M. J. (Mikael J.), Hellberg, I. (Iida), Turkiewicz, A. (Aleksandra), Lutz-Bueno, V. (Viviane), Jonsson, E. (Elin), Holler, M. (Mirko), Ali, N. (Neserin), Hughes, V. (Velocity), Isaksson, H. (Hanna), Tjörnstrand, J. (Jon), Önnerfjord, P. (Patrik), Guizar-Sicairos, M. (Manuel), Saarakkala, S. (Simo), Englund, M. (Martin) |
| بيانات النشر: |
John Wiley & Sons |
| سنة النشر: |
2022 |
| المجموعة: |
Jultika - University of Oulu repository / Oulun yliopiston julkaisuarkisto |
| مصطلحات موضوعية: |
ANALYSIS/QUANTITATION OF BONE, BONE MODELING AND REMODELING, COLLAGEN, MATRIX MINERALIZATION, OSTEOARTHRITIS |
| الوصف: |
Osteoarthritis (OA) is the most common joint disease, where articular cartilage degradation is often accompanied with sclerosis of the subchondral bone. However, the association between OA and tissue mineralization at the nanostructural level is currently not understood. In particular, it is technically challenging to study calcified cartilage, where relevant but poorly understood pathological processes such as tidemark multiplication and advancement occur. Here, we used state-of-the-art microfocus small-angle X-ray scattering with a 5-μm spatial resolution to determine the size and organization of the mineral crystals at the nanostructural level in human subchondral bone and calcified cartilage. Specimens with a wide spectrum of OA severities were acquired from both medial and lateral compartments of medial compartment knee OA patients (n = 15) and cadaver knees (n = 10). Opposing the common notion, we found that calcified cartilage has thicker and more mutually aligned mineral crystals than adjoining bone. In addition, we, for the first time, identified a well-defined layer of calcified cartilage associated with pathological tidemark multiplication, containing 0.32 nm thicker crystals compared to the rest of calcified cartilage. Finally, we found 0.2 nm thicker mineral crystals in both tissues of the lateral compartment in OA compared with healthy knees, indicating a loading-related disease process because the lateral compartment is typically less loaded in medial compartment knee OA. In summary, we report novel changes in mineral crystal thickness during OA. Our data suggest that unloading in the knee might be involved with the growth of mineral crystals, which is especially evident in the calcified cartilage. © 2022 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR). |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
info:eu-repo/grantAgreement/EC/H2020/713645/EU/Biomedical Engineering and Medical Physics/BioMEP; info:eu-repo/grantAgreement/EC/FP7/336267/EU/Development of 3D Histopathological Grading of Osteoarthritis/3D-OA-HISTO |
| الإتاحة: |
http://urn.fi/urn:nbn:fi-fe2023061956422Test |
| حقوق: |
info:eu-repo/semantics/openAccess ; © 2022 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR). This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. ; https://creativecommons.org/licenses/by-nc-nd/4.0Test/ |
| رقم الانضمام: |
edsbas.69CFD5E |
| قاعدة البيانات: |
BASE |
