دورية أكاديمية
Metabolic changes in mouse sperm during capacitation†
| العنوان: | Metabolic changes in mouse sperm during capacitation† |
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| المؤلفون: | Balbach, Melanie, Gervasi, Maria Gracia, Hidalgo, David Martin, Visconti, Pablo E, Levin, Lonny R, Buck, Jochen |
| المساهمون: | Fondo Social Europeo, National Institute of Child Health and Human Development |
| المصدر: | Biology of Reproduction ; volume 103, issue 4, page 791-801 ; ISSN 0006-3363 1529-7268 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2020 |
| الوصف: | Mammalian sperm are stored in the epididymis in a dormant state. Upon ejaculation, they must immediately start producing sufficient energy to maintain motility and support capacitation. While this increased energy demand during capacitation is well established, it remains unclear how mouse sperm modify their metabolism to meet this need. We now show that capacitating mouse sperm enhance glucose uptake, identifying glucose uptake as a functional marker of capacitation. Using an extracellular flux analyzer, we show that glycolysis and oxidative phosphorylation increase during capacitation. Furthermore, this increase in oxidative phosphorylation is dependent on glycolysis, providing experimental evidence for a link between glycolysis and oxidative phosphorylation in mouse sperm. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1093/biolre/ioaa114 |
| DOI: | 10.1093/biolre/ioaa114/33569763/ioaa114.pdf |
| الإتاحة: | https://doi.org/10.1093/biolre/ioaa114Test http://academic.oup.com/biolreprod/article-pdf/103/4/791/35974003/ioaa114.pdfTest |
| حقوق: | https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_modelTest |
| رقم الانضمام: | edsbas.66C6F5B2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/biolre/ioaa114 |
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