In vitro EROD induction equivalency factors for the 10 PAHs generally monitored in risk assessment studies in The Netherlands
| العنوان: | In vitro EROD induction equivalency factors for the 10 PAHs generally monitored in risk assessment studies in The Netherlands |
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| المؤلفون: | Hester Jongepier, Anette V. Klomp, Paul A.F. de Bie, Nico W. van den Brink, A.T.C. Bosveld |
| المصدر: | Chemosphere 49 (2002) 1 Chemosphere, 49(1), 75-83 |
| بيانات النشر: | Elsevier BV, 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | Chrysene, Environmental Engineering, milieu, Stereochemistry, Health, Toxicology and Mutagenesis, Models, Biological, Risk Assessment, chemistry.chemical_compound, Toxicity Tests, Cytochrome P-450 CYP1A1, polycyclic compounds, Animals, Humans, Environmental Chemistry, Potency, Bioassay, Receptors, Cholinergic, Polycyclic Aromatic Hydrocarbons, Toxic equivalency factor, Cells, Cultured, Carcinogen, Netherlands, Fluoranthene, Dose-Response Relationship, Drug, Chemistry, Ecologie en Milieu, biochemie, ecotoxicologie, Public Health, Environmental and Occupational Health, General Medicine, General Chemistry, Phenanthrene, Pollution, Diet, Rats, Environmental chemistry, Pyrene, Environmental Monitoring |
| الوصف: | The ethoxy resorufin dealkylase (EROD) inducing potency of 10 polycyclic aromatic hydrocarbons (PAHs) is measured in the H4IIE in vitro bioassay and the results are compared to those reported in literature. The selected PAHs varied considerably in their potency to induce EROD activity. Anthracene (Ant) and phenanthrene (Phe) showed consistently no response. Naphthalene (Nap) showed no or a very weak response on EROD activity. Fluoranthene (Fla) and benzo[g,h,i]perylene (BghiP) showed weak responses at the highest doses. The other PAHs, including indeno[1,2,3-cd]pyrene (IP), benz[a]anthracene (BaA), benzo[a]pyrene (BaP), chrysene (Chr) and benzo[k]fluoranthene (BkF), showed full bell shaped dose-response curves. BaP EROD induction equivalency factors (BaP-1EF) were calculated and increased in the order Ant approximately PheFlaNapBghiPIPBaABaPChrBkF. Comparison of BaP-IEFs based on 50% effect concentration (EC50) or lowest effect concentration (LEC), yielded a significant relationship between both methods described by the equation log(BaPIEF(EC50) = 0.55 x log(BaPIEF(LEC)) + 0.07 (r2 = 0.913). BaP-IEFs as derived from our measurements and as reported in literature and measured in other in vitro assays deviated up to a factor of 17 among the different studies, but the potency rankings were comparable. For the PAH mixture as on average present in the human diet an overall tetrachlorodibenzo-p-dioxin (TCDD)-IEF of 1 x 10(-4) was estimated. The total PAH based TCDD induction equivalents (IEQ) intake then was calculated 300 pg/day, which is approximately 2 times higher then the PHAH based TCDD-EQ intake reported for humans. |
| وصف الملف: | application/pdf; application/octet-stream |
| تدمد: | 0045-6535 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a944a28c4c7fb0cb7d3880a1d47c5d5Test https://doi.org/10.1016/s0045-6535Test(02)00161-3 |
| حقوق: | RESTRICTED |
| رقم الانضمام: | edsair.doi.dedup.....9a944a28c4c7fb0cb7d3880a1d47c5d5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00456535 |
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