دورية أكاديمية
Impact of hydroxytyrosol on stroke: tracking therapy response on neuroinflammation and cerebrovascular parameters using PET-MR imaging and on functional outcomes
| العنوان: | Impact of hydroxytyrosol on stroke: tracking therapy response on neuroinflammation and cerebrovascular parameters using PET-MR imaging and on functional outcomes |
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| المؤلفون: | Barca, Cristina, Wiesmann, Maximilian, Calahorra, Jesús, Wachsmuth, Lydia, Döring, Christian, Foray, Claudia, Heiradi, Ali, Hermann, Sven, Ángeles Peinado, Maria, Siles, Eva, Faber, Cornelius, Schäfers, Michael, Kiliaan, Amanda J, Jacobs, Andreas H, Zinnhardt, B |
| سنة النشر: | 2021 |
| المجموعة: | Zenodo |
| مصطلحات موضوعية: | Hydroxytyrosol, transient middle artery occlusion, neuroinflammation, [18]DPA-714, multimodal imaging, TSPO |
| الوصف: | Immune cells have been implicated in influencing stroke outcomes depending on their temporal dynamics, number, and spatial distribution after ischemia. Depending on their activation status, immune cells can have detrimental and beneficial properties on tissue outcome after stroke, highlighting the need to modulate inflammation towards beneficial and restorative immune responses. Novel dietary therapies may promote modulation of pro- and anti-inflammatory immune cell functions. Among the dietary interventions inspired by the Mediterranean diet, hydroxytyrosol (HT), the main phenolic component of the extra virgin olive oil (EVOO), has been suggested to have antioxidant and anti-inflammatory properties in vitro. However, immunomodulatory effects of HT have not yet been studied in vivo after stroke. The aim of this project is therefore to monitor the therapeutic effect of a HT-enriched diet in an experimental stroke model using non-invasive in vivo multimodal imaging, behavioural phenotyping and cross-correlation with ex vivo parameters. Methods: A total of N = 22 male C57BL/6 mice were fed with either a standard chow (n = 11) or a HT enriched diet (n = 11) for 35 days, following a 30 min transient middle cerebral artery occlusion (tMCAo). T2-weighted (lesion) and perfusion (cerebral blood flow)-/diffusion (cellular density)-weighted MR images were acquired at days 1, 3, 7, 14, 21 and 30 post ischemia. [18F]DPA-714 (TSPO, neuroinflammation marker) PET-CT scans were acquired at days 7, 14, 21 and 30 post ischemia. Infarct volume (mm3), cerebral blood flow (mL/100g/min), apparent diffusion coefficient (10-4·mm2/s) and percentage of injected tracer dose (%ID/mL) were assessed. Behavioural tests (grip test, rotarod, open field, pole test) were performed prior and after ischemia to access therapy effects on sensorimotor functions. Ex vivo analyses (IHC, IF, WB) were performed to quantify TSPO expression, immune cells including microglia/macrophages (Iba-1, F4/80), astrocytes (GFAP) and peripheral markers in serum such as ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/grantAgreement/EC/H2020/675417/; info:eu-repo/grantAgreement/EC/H2020/831514/; https://zenodo.org/communities/immune-imageTest; https://zenodo.org/record/5336478Test; https://doi.org/10.7150/thno.48110Test; oai:zenodo.org:5336478 |
| DOI: | 10.7150/thno.48110 |
| الإتاحة: | https://doi.org/10.7150/thno.48110Test https://zenodo.org/record/5336478Test |
| حقوق: | info:eu-repo/semantics/openAccess ; https://creativecommons.org/licenses/by/4.0/legalcodeTest |
| رقم الانضمام: | edsbas.8040CE39 |
| قاعدة البيانات: | BASE |
| DOI: | 10.7150/thno.48110 |
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