Th2 Dominance of T Helper Cell Response to Preproinsulin in Individuals with Preclinical Type 1 Diabetes
| العنوان: | Th2 Dominance of T Helper Cell Response to Preproinsulin in Individuals with Preclinical Type 1 Diabetes |
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| المؤلفون: | Bernhard O. Boehm, Hans-Jürgen Schreckling, Silke Rosinger, P. Kuehnl, Ivana Durinovic-Belló, Michael Schlosser, Hubert Kalbacher, Martina Riedl, Martin Deeg, Manfred Ziegler, Nicola Maisel |
| المصدر: | Annals of the New York Academy of Sciences. 958:209-213 |
| بيانات النشر: | Wiley, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Adult, Male, Preproinsulin, medicine.medical_specialty, Diabetes risk, Adolescent, medicine.medical_treatment, Biology, Peripheral blood mononuclear cell, General Biochemistry, Genetics and Molecular Biology, Th2 Cells, History and Philosophy of Science, HLA Antigens, immune system diseases, Internal medicine, medicine, Humans, Insulin, Protein Precursors, Child, Cells, Cultured, Protein Tyrosine Phosphatase, Non-Receptor Type 1, Type 1 diabetes, General Neuroscience, T helper cell, Middle Aged, medicine.disease, Diabetes Mellitus, Type 1, Endocrinology, Cytokine, medicine.anatomical_structure, Child, Preschool, Immunology, Leukocyte Common Antigens, Female, Cytokine secretion, Interleukin-4, Interleukin-5, Immunologic Memory, Proinsulin |
| الوصف: | In human type 1 diabetes (T1D) autoantibodies to insulin precede clinical disease, while little is known about the contribution of insulin-specific T lymphocytes-in particular, T helper (Th) subsets. Here we have studied the in vivo primed cytokine response to preproinsulin in peripheral blood mononuclear cells (PBMCs) and two major Th cell subsets-CD45RO+ memory cells and CD45RA+ naive/resting cells-in 35 individuals with HLA-DRB1*04, DQB1*0302 diabetes risk marker: 12 patients with T1D, 12 autoantibody-positive (Ab+) individuals, and 11 healthy controls. Cytokine secretion (TNF-alpha, IFN-gamma, IL-2, IL-4, IL-5, and IL-10) was measured in the supernatants of the cultures stimulated with 21 overlapping preproinsulin peptides as well as proinsulin and insulin. In Ab+ individuals our results reveal higher IL-4 levels in CD45RO+ memory cells and higher IL-5 levels in CD45RA+ naive/resting cells, while higher IL-2 production was found in PBMCs. In contrast, in PBMCs of T1D patients higher IFN-gamma and IL-10 secretion was found. Our data delineate characteristic cytokine patterns in peripheral T lymphocytes from patients at different stages of the T1D development. |
| تدمد: | 1749-6632 0077-8923 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0c90920db9fad1c3fbc6c8605dc7222Test https://doi.org/10.1111/j.1749-6632.2002.tb02971.xTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c0c90920db9fad1c3fbc6c8605dc7222 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17496632 00778923 |
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