كتاب
Organic consequences of ileal transposition in rats with diet-induced obesity
| العنوان: | Organic consequences of ileal transposition in rats with diet-induced obesity |
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| المؤلفون: | Lucas P. F. F. Leal, Joao Luiz M. C. Azevedo, Gilmara S. Aguiar-Yamaguchi, Wellington Cardia, Bianca Marigliani, Karina F. Neves, Paolo H. B. Lourenço, Marcelo C. Carlini, Joao L. C. Azevedo, Otávio C. Azevedo, Gui M. Ko, Gustavo P. S. Miguel, Valderez B. V. Lapchick |
| المصدر: | Nature Precedings |
| سنة النشر: | 2011 |
| المجموعة: | Nature Precedings |
| مصطلحات موضوعية: | Developmental Biology, Genetics & Genomics, Molecular Cell Biology |
| الوصف: | INTRODUCTION: The clinical management of metabolic syndrome - especially diabetes mellitus type 2 - is notoriously complex due to the progressive nature of this disease. At present, there is a need for a surgical procedure that is effective for the treatment of diabetes mellitus type 2, even in non-obese individuals. The isolated ileal transposition theory could lead to an effective alternative therapy. This intervention has not yet been performed in humans, and there are no reports of its use in an experimental model of diet-induced metabolic syndrome. OBJECTIVES: The objective of this study is to evaluate the physiological effects of ileal transposition in rats with diet-induced metabolic syndrome. The effects of this procedure on glucose and lipid metabolism will be assessed. METHODS: Forty 12-week-old male rats (albino Rattus norvegicus, Wistar, 2BAW, heterogeneous) will be divided into four groups of 10 animals each: the ileal transposition group (TG) comprising animals on a hypercaloric-hyperlipidic diet; the sham group (SG) containing animals that receive the same diet and undergo the sham surgery; control group 1 (CG1), which will receive a hypercaloric-hyperlipidic diet and will not undergo surgery; and control group 2 (CG2), which will consume standard feed and will not undergo surgery. The surgeries will be performed in 20-week-old animals. Blood samples for laboratory testing will be collected from 12-week-old animals on the day of surgery and after eight postoperative weeks, following determination of the weights of the animals and the administration of anesthesia. The levels of serum glucose, insulin, triglycerides, total cholesterol and fractions, glucagon-like peptide-1, C-peptide and glycated hemoglobin will be assessed in all of the animals. The insulin tolerance test will be performed using PRISMA software, and insulin resistance will be calculated by the HOMA-IR indirect test. On specific days, two 20-week-old rats will be separated and randomly distributed ... |
| نوع الوثيقة: | manuscript |
| اللغة: | unknown |
| العلاقة: | http://precedings.nature.com/documents/6122/version/1Test; oai:nature.com:10.1038/npre.2011.6122.1; http://dx.doi.org/10.1038/npre.2011.6122.1Test |
| DOI: | 10.1038/npre.2011.6122.1 |
| الإتاحة: | https://doi.org/10.1038/npre.2011.6122.1Test http://precedings.nature.com/documents/6122/version/1Test |
| حقوق: | Creative Commons Attribution 3.0 License |
| رقم الانضمام: | edsbas.E8A6C6A4 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/npre.2011.6122.1 |
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