Cross-presentation and genome-wide screening reveal candidate T cells antigens for a herpes simplex virus type 1 vaccine
| العنوان: | Cross-presentation and genome-wide screening reveal candidate T cells antigens for a herpes simplex virus type 1 vaccine |
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| المؤلفون: | David M. Koelle, Jürgen Haas, Georges M. G. M. Verjans, Christopher L. McClurkan, Joseph J. Bruckner, Anna Wald, Lichun Dong, Tori N. Yamamoto, Susanne M. Bailer, Joshua O. Marshak, Lichen Jing, Tiana M. Chong, Greg C. Dann, Kerry J. Laing |
| المساهمون: | Publica, Virology |
| المصدر: | Jing, L, Haas, J, Chong, T M, Bruckner, J J, Dann, G C, Dong, L, Marshak, J O, McClurkan, C L, Yamamoto, T N, Bailer, S M, Laing, K J, Wald, A, Verjans, G M G M & Koelle, D M 2012, ' Cross-presentation and genome-wide screening reveal candidate T cells antigens for a herpes simplex virus type 1 vaccine ', Journal of Clinical Investigation, vol. 122, no. 2, pp. 654-673 . https://doi.org/10.1172/JCI60556Test Journal of Clinical Investigation, 122(2), 654-673. The American Society for Clinical Investigation |
| بيانات النشر: | American Society for Clinical Investigation, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Adult, CD4-Positive T-Lymphocytes, Cytotoxicity, Immunologic, Male, viruses, T cell, Herpesvirus 1, Human, Human leukocyte antigen, Streptamer, CD8-Positive T-Lymphocytes, Biology, medicine.disease_cause, Genome, Interferon-gamma, Tumor Necrosis Factor Receptor Superfamily, Member 9, Young Adult, Cross-Priming, SDG 3 - Good Health and Well-being, Antigen, HLA Antigens, Clinical investigation, medicine, Humans, Antigens, Viral, Pan-T antigens, Cells, Cultured, Medicine(all), Viral Vaccine, Cross-presentation, Herpes Simplex, Viral Vaccines, General Medicine, Middle Aged, Virology, Herpes simplex virus, medicine.anatomical_structure, Technical Advance, Immunology, Female, Corrigendum, CD8 |
| الوصف: | Herpes simplex virus type 1 (HSV-1) not only causes painful recurrent oral-labial infections, it can also cause permanent brain damage and blindness. There is currently no HSV-1 vaccine. An effective vaccine must stimulate coordinated T cell responses, but the large size of the genome and the low frequency of HSV-1-specific T cells have hampered the search for the most effective T cell antigens for inclusion in a candidate vaccine. We have now developed what we believe to be novel methods to efficiently generate a genome-wide map of the responsiveness of HSV-1-specific T cells, and demonstrate the applicability of these methods to a second complex microbe, vaccinia virus. We used cross-presentation and CD 137 activation-based FACS to enrich for polyclonal CD8(+) T effector T cells. The HSV-1 proteome was prepared in a flexible format for analyzing both CD8(+) and CD4(+) T cells from study participants. Scans with participant-specific panels of artificial APCs identified an oligospecific response in each individual. Parallel CD137-based CD4(+) T cell research showed discrete oligospecific recognition of HSV-1 antigens. Unexpectedly, the two HSV-1 proteins not previously considered as vaccine candidates elicited both CD8(+) and CD4(+) T cell responses in most HSV-1-infected individuals. In this era of microbial genomics, our methods also - demonstrated in principle for vaccinia virus for both CD8(+) and CD4(+) T cells - should be broadly applicable to the selection of T cell antigens for inclusion in candidate vaccines for many pathogens. |
| وصف الملف: | application/pdf |
| تدمد: | 0021-9738 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a769c9727dc1284d26cc58bfadeb7ba7Test https://doi.org/10.1172/jci60556Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a769c9727dc1284d26cc58bfadeb7ba7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00219738 |
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