دورية أكاديمية
Plant tissue culture independent Agrobacterium tumefaciens mediated In-planta transformation strategy for upland cotton (Gossypium hirsutum)
| العنوان: | Plant tissue culture independent Agrobacterium tumefaciens mediated In-planta transformation strategy for upland cotton (Gossypium hirsutum) |
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| المؤلفون: | Bipinchandra B. Kalbande, Anita S. Patil |
| المصدر: | Journal of Genetic Engineering and Biotechnology, Vol 14, Iss 1, Pp 9-18 (2016) |
| بيانات النشر: | Elsevier, 2016. |
| سنة النشر: | 2016 |
| المجموعة: | LCC:Biotechnology LCC:Genetics |
| مصطلحات موضوعية: | In-planta transformation, Diploid cotton, Gossypium hirsutum cv LRK-516 Anjali, pBinAR, At-NPR1 gene, Cotyledonary leaves, Meristem cells, Transformation frequency, Biotechnology, TP248.13-248.65, Genetics, QH426-470 |
| الوصف: | A new method of transgenic development called “In-planta” transformation method, where Agrobacterium is used to infect the plantlets but the steps of in vitro regeneration of plants is totally avoided. In this study, we have reported a simple In-planta method for efficient transformation of diploid cotton Gossypium hirsutum cv LRK-516 Anjali using Agrobacterium tumefaciens EHA-105 harbouring recombinant binary vector plasmid pBinAR with Arabidopsis At-NPR1 gene. Four day old plantlets were used for transformation. A vertical cut was made at the junction of cotyledonary leaves, moderately bisecting the shoot tip and exposing meristem cells at apical meristem. This site was infected with Agrobacterium inoculum. The transgenic events obtained were tested positive for the presence of At-NPR1 gene with promoter nptII gene. They are also tested negative for vector backbone integration and Agrobacterium contamination in T0 events. With this method a transformation frequency of 6.89% was reported for the cv LRK-516. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1687-157X |
| العلاقة: | http://www.sciencedirect.com/science/article/pii/S1687157X16300105Test; https://doaj.org/toc/1687-157XTest |
| DOI: | 10.1016/j.jgeb.2016.05.003 |
| الوصول الحر: | https://doaj.org/article/2eff9007f3ae467bb7f95c432e8c5de8Test |
| رقم الانضمام: | edsdoj.2eff9007f3ae467bb7f95c432e8c5de8 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 1687157X |
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| DOI: | 10.1016/j.jgeb.2016.05.003 |