Comprehensive epigenome characterization reveals diverse transcriptional regulation across human vascular endothelial cells
| العنوان: | Comprehensive epigenome characterization reveals diverse transcriptional regulation across human vascular endothelial cells |
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| المؤلفون: | Nakato, R., Wada, Y., Nakaki, R., Nagae, G., Katou, Y., Tsutsumi, S., Nakajima, N., Fukuhara, H., Iguchi, A., Kohro, T., Kanki, Y., Saito, Y., Kobayashi, M., Izumi-Taguchi, A., Osato, N., Tatsuno, K., Kamio, A., Hayashi-Takanaka, Y., Wada, H., Ohta, S., Aikawa, M., Nakajima, H., Nakamura, M., McGee, R. C., Heppner, K. W., Kawakatsu, T., Genno, M., Yanase, H., Kume, H., Senbonmatsu, T., Homma, Y., Nishimura, S., Mitsuyama, T., Aburatani, H., Kimura, Hiroshi, Shirahige, K. |
| المصدر: | Epigenetics & Chromatin, Vol 12, Iss 1, Pp 1-16 (2019) Epigenetics & Chromatin |
| بيانات النشر: | BMC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | lcsh:QH426-470, Angiogenesis, Endothelial cells, Computational biology, Biology, ChIP-seq, Histone modifications, Transcriptome, Histones, 03 medical and health sciences, Epigenome, Epigenome database, 0302 clinical medicine, Vasculogenesis, Databases, Genetic, Genetics, Transcriptional regulation, Humans, Enhancer, Hox gene, Promoter Regions, Genetic, Molecular Biology, Large-scale analysis, 030304 developmental biology, Epigenomics, Homeodomain Proteins, 0303 health sciences, Principal Component Analysis, Research, Phenotype, Chromatin, Cell biology, Histone Code, lcsh:Genetics, Enhancer Elements, Genetic, Gene Expression Regulation, Homeobox, 030217 neurology & neurosurgery, Genome-Wide Association Study |
| الوصف: | BackgroundEndothelial cells (ECs) make up the innermost layer throughout the entire vasculature. Their phenotypes and physiological functions are initially regulated by developmental signals and extracellular stimuli. The underlying molecular mechanisms responsible for the diverse phenotypes of ECs from different organs are not well understood.ResultsTo characterize the transcriptomic and epigenomic landscape in the vascular system, we cataloged gene expression and active histone marks in nine types of human ECs (generating 148 genome-wide datasets) and carried out a comprehensive analysis with chromatin interaction data. We developed a robust procedure for comparative epigenome analysis that circumvents variations at the level of the individual and technical noise derived from sample preparation under various conditions. Through this approach, we identified 3765 EC-specific enhancers, some of which were associated with disease-associated genetic variations. We also identified various candidate marker genes for each EC type. We found that the nine EC types can be divided into two subgroups, corresponding to those with upper-body origins and lower-body origins, based on their epigenomic landscape. Epigenomic variations were highly correlated with gene expression patterns, but also provided unique information. Most of the deferentially expressed genes and enhancers were cooperatively enriched in more than one EC type, suggesting that the distinct combinations of multiple genes play key roles in the diverse phenotypes across EC types. Notably, many homeobox genes were differentially expressed across EC types, and their expression was correlated with the relative position of each organ in the body. This reflects the developmental origins of ECs and their roles in angiogenesis, vasculogenesis and wound healing.ConclusionsThis comprehensive analysis of epigenome characterization of EC types reveals diverse transcriptional regulation across human vascular systems. These datasets provide a valuable resource for understanding the vascular system and associated diseases. |
| اللغة: | English |
| تدمد: | 1756-8935 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce2571d3a0ad767c4a265f600de947c9Test https://doaj.org/article/360a125b620f4aad860552d5cf426796Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ce2571d3a0ad767c4a265f600de947c9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17568935 |
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