دورية أكاديمية
Regions of the varicella-zoster virus open reading frame 63 latency-associated protein important for replication in vitro are also critical for efficient establishment of latency.
| العنوان: | Regions of the varicella-zoster virus open reading frame 63 latency-associated protein important for replication in vitro are also critical for efficient establishment of latency. |
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| المؤلفون: | Cohen, Jeffrey I, Krogmann, Tammy, Bontems, Sébastien, Sadzot-Delvaux, Catherine, Pesnicak, Lesley |
| المصدر: | Journal of Virology, 79 (8), 5069-77 (2005) |
| بيانات النشر: | American Society for Microbiology |
| سنة النشر: | 2005 |
| المجموعة: | University of Liège: ORBi (Open Repository and Bibliography) |
| مصطلحات موضوعية: | Animals, Cell Line, Tumor, Cell Nucleus/virology, Cloning, Molecular, Cosmids, Ganglia/virology, Herpesvirus 3, Human/genetics/growth & development/physiology, Humans, Immediate-Early Proteins/genetics, Melanoma, Mutagenesis, Open Reading Frames/genetics, Phosphorylation, Restriction Mapping, Sigmodontinae, Transcription, Genetic, Viral Envelope Proteins/genetics, Virus Latency/genetics, Virus Replication/genetics, Life sciences, Biochemistry, biophysics & molecular biology, Sciences du vivant, Biochimie, biophysique & biologie moléculaire |
| الوصف: | peer reviewed ; Varicella-zoster virus (VZV) open reading frame 63 (ORF63) is one of the most abundant transcripts expressed during VZV latency in humans, and ORF63 protein has been detected in human ganglia by several laboratories. Deletion of over 90% of the ORF63 gene showed that the protein is required for efficient establishment of latency in rodents. We have constructed viruses with a series of mutations in ORF63. While prior experiments showed that transfection of cells with a plasmid expressing ORF63 but lacking the putative nuclear localization signal of the protein resulted in increased expression of the protein in the cytoplasm, we found that ORF63 protein remained in the nucleus in cells infected with a VZV ORF63 nuclear localization signal deletion mutant. This mutant was not impaired for growth in cell culture or for latency in rodents. Replacement of five serine or threonine phosphorylation sites in ORF63 with alanines resulted in a virus that was impaired for replication in vitro and for latency. A series of ORF63 carboxy-terminal mutants showed that the last 70 amino acids do not affect replication in vitro or latency in rodents; however, the last 108 amino acids are important for replication and latency. Thus, regions of ORF63 that are important for replication in vitro are also required for efficient establishment of latency. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0022-538X 1098-5514 |
| العلاقة: | urn:issn:0022-538X; urn:issn:1098-5514; https://orbi.uliege.be/handle/2268/1637Test; info:hdl:2268/1637; https://orbi.uliege.be/bitstream/2268/1637/1/Cohen%2063%3a10M.pdfTest; scopus-id:2-s2.0-16244395803; info:pmid:15795292 |
| DOI: | 10.1128/JVI.79.8.5069-5077.2005 |
| الإتاحة: | https://doi.org/10.1128/JVI.79.8.5069-5077.2005Test https://orbi.uliege.be/handle/2268/1637Test https://orbi.uliege.be/bitstream/2268/1637/1/Cohen%2063%3a10M.pdfTest |
| حقوق: | open access ; http://purl.org/coar/access_right/c_abf2Test ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.50C7BD79 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 0022538X 10985514 |
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| DOI: | 10.1128/JVI.79.8.5069-5077.2005 |